Screening of bacteria producing acid-stable and thermostable endo - 1,4 -β-glucanase from hot springs of North and Northwest of Iran
عنوان مقاله: Screening of bacteria producing acid-stable and thermostable endo - 1,4 -β-glucanase from hot springs of North and Northwest of Iran
شناسه ملی مقاله: JR_ARMMT-1-1_003
منتشر شده در شماره 1 دوره 1 فصل در سال 1397
شناسه ملی مقاله: JR_ARMMT-1-1_003
منتشر شده در شماره 1 دوره 1 فصل در سال 1397
مشخصات نویسندگان مقاله:
Hasan Diba - Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box ۳۳۵۳۵۱۱۱, Tehran, Iran
Jafar Hemmat - Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box ۳۳۵۳۵۱۱۱, Tehran, Iran
Mohsen Vaez - Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box ۳۳۵۳۵۱۱۱, Tehran, Iran
Mohammad Amoozegar - Extremophile lab. Dept. microbiology, school of biology, college of science,university of Tehran, Iran
خلاصه مقاله:
Hasan Diba - Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box ۳۳۵۳۵۱۱۱, Tehran, Iran
Jafar Hemmat - Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box ۳۳۵۳۵۱۱۱, Tehran, Iran
Mohsen Vaez - Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box ۳۳۵۳۵۱۱۱, Tehran, Iran
Mohammad Amoozegar - Extremophile lab. Dept. microbiology, school of biology, college of science,university of Tehran, Iran
Cellulases with capability to have enzymatic functions at low pH and high temperature are important and include considerable portion of industrial enzymes. Endo -1,4 -β-glucanase is one of the three cellulolytic enzymes in a triplet catalytic system that required for extracellular cellulose hydrolysis. In this study, the sampling was performed from four hot springs in north and northwest of Iran and the screening and identification of acid-stable and thermostable of endo -1,4 -β-glucanase producing bacteria were investigated. Endo -1,4 -β-glucanase of the isolated strains were determined by qualitative Congo-Red staining as well as quantitative Carboxymethylcellulose/Dinitrosalicylic acid methods as indicators of cellulase activity. Three isolates out of twelve initially selected bacteria, showed noticeable endo -1,4 -β-glucanase activity, including Paenibacillus sp. ASh 4 , Bacillus sp. AGh 1 and Bacillus sp. AG 2 with 90 %, 77 % and 45 % residual activity at pH= 4 and 60 oC after three hours. Molecular identification of the bacteria was carried out using 16 S rDNA partial sequencing, in which two isolates belonged to Bacillus sp. and one to Paenibacillus sp.. The results shown that the isolated acido-thermotolerant Bacillus spp. and Paenibacillus sp. had the capability to produce proper acid-stable and thermostable endoglucanase. These isolate also may be even considering as candidates for satisfactory production for other thermostable metabolites with further biotechnological applications .
کلمات کلیدی: Cellulase, Thermostability, Hot spring, Acidostability
صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/868567/