Sana Rabiei - Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran
Masoud Rezaie - Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran
Zahra Abasian - Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Mohammad Khezri - Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran

Objective(s): Today, consumers are looking for food products providing health benefits in addition to meeting the basic nutritional needs of the body. This study aimed to evaluate the antioxidant and cytotoxic effects of Liza klunzingeri protein hydrolysate both in vivo and in vitro.Materials and Methods: Fish protein hydrolysate (FPH) was prepared using enzymatic hydrolysis with papain. In vitro antioxidant activity was assessed using five different antioxidant assays. The cytotoxic effect on 4T1 cell line was evaluated using the MTT assay. The distribution of the molecular weight of FPH was measured using HPLC. In the in vivo study, CCl4-exposed Wistar rats were orally treated with FPH (150, 300, and 600 mg/kg) or gallic acid (50 mg/kg) for 28 consecutive days.Results: Enzymatic hydrolysis gave hydrolysate rich in low molecular weight peptides (Conclusion: L. klunzingeri protein hydrolysate can be considered as a functional food to alleviate oxidative stress.

Antioxidant activity, Cytotoxicity, Liza klunzingeri, Oxidative stress, Protein hydrolysate