Yiping Lin - Jinhua Polytechnic, Jinhua ۳۲۱۰۰۷, Zhejiang, China
Yanli Wei - Jinhua Polytechnic, Jinhua ۳۲۱۰۰۷, Zhejiang, China
Xiaoxia Hu - Jinhua Central Hospital, Jinhua ۳۲۱۰۰۰, Zhejiang, China
Mei-Ling Wu - Jinhua Polytechnic, Jinhua ۳۲۱۰۰۷, Zhejiang, China

Objective(s): In this study, a cocktail of probe drugs was used to assess whether lentinan could influence the activities of rat enzymes CYP3A4, CYP2D6, CYP1A2, CYP2C19, and CYP2C9 in vivo. Materials and Methods: Fourteen days after intraperitoneal injection of lentinan, rats were given an oral dose of a cocktail solution containing phenacetin, tolbutamide, omeprazole, metoprolol, and midazolam. Then, we obtained blood in specific durations for the determination of plasma concentration of the probe drugs using UPLC-MS/MS. We also evaluated the pharmacokinetic parameters using the DAS 2.0 software. Results: We found that various concentrations of lentinan increased the activity of rat CYP1A2, CYP3A4, CYP2D6, and CYP2C19 but not CYP2C9. Conclusion: These findings suggest that clinical application of lentinan combination with CYP3A4, CYP1A2, CYP2C19, or CYP2D6 should be given careful consideration as this may lead to herb-drug interactions and hence treatment failure.

Cocktail, CYP, Herb-drug interaction, Lentinan, Probe drug