Nor Asyiqin Bakeri - Department of Bioprocess and Polymer Engineering, Universiti Teknologi Malaysia
Mohd Amzar Mohamed Zahari - Department of Bioprocess and Polymer Engineering, Universiti Teknologi Malaysia
Firdaus Kamarulzaman - Natural Product Division, Forest Research Institute Malaysia (FRIM)
Nohaniza Yusof - Advanced Membrane Technology Research Centre (AMT, Universiti Teknologi Malaysia

This study analyzed the inhibition activity of Swietenia mahagoni seeds extract on α-glucosidase and α-amylase enzymes inhibition assays.  Swietenia mahagoni seeds were extracted by using Supercritical Carbon Dioxide (SC-CO2) extraction at pressures of 20- 30 MPa and temperatures of 40- 60°C.  The oil yields obtained were analyzed with α- glucosidase and α- amylase enzymes inhibition assays.  All data obtained were expressed as mean ± standard deviation for triplicate experiments.  One way analysis was used for statistical significance by using Statistica software version 7.0 (StartSoft, EUA) and IC50 (extract concentration causing 50% enzyme inhibitory) was determined by using GraphPad Prism 6.0 software.  Swietenia mahagoni seeds extract have a strong inhibition of α-glucosidase enzyme activity (98.4% ± 0.2) but a moderate inhibition of α- amylase enzyme activity (34.9% ± 1.2). These findings implied that Swietenia mahagoni seeds extract could be an effective natural antidiabetic agent.

Swietenia mahagoni seed, α, glucosidase enzyme, amylase enzyme, Supercritical Carbon Dioxide (SC, CO2) extraction