Majid Sartipi - Assistant Professor, School of Health, Zahedan University of Medical Sciences, Zahedan, Iran.
Mehdi Zanganeh Baygi - Assistant Professor, School of Health, Zahedan University of Medical Sciences, Zahedan, Iran.
Seyed Mehdi Tabatabaei - Assistant Professor, School of Health, Zahedan University of Medical Sciences, Zahedan, Iran.
Hamid Azarian-Moghadam - MSc, Center for Disease Control and Prevention, Deputy of Health, Zahedan University of Medical Sciences, Zahedan, Iran.Tehran, Iran
Abdolghafar Hasanzehi - MSc, Center for Disease Control and Prevention, Deputy of Health, Zahedan University of Medical Sciences, Zahedan, Iran.Tehran, Iran
Ali Naghizadeh - MSc, Center for Disease Control and Prevention, Deputy of Health, Zahedan University of Medical Sciences, Zahedan, Iran.Tehran, Iran

Background: Lucilia sericata larvae have been successfully used as medicinal larvae for wound healing. This study shows that excretory secretory substances of larvae effectively help the wound healing process.Objectives: This study was performed to evaluate the therapeutic activity of primary ointment made from excretory-secretory substances of Lucilia sericata larvae on experimental burn wounds on rabbits in laboratory conditions.Materials and Methods: extraction of ES from the third stage of Lucicia sericata larvae and antibiogram testing was done in order. Four rabbits were randomly divided into four groups and then under general anesthesia, three standard third grade burn wounds were created on the back of each rabbit. For intervention groups (B, D) of wounds were used respectively from the original ointment made from ES and ES without Accompanying material. Nitrofurazone ointment was used for the positive lortnoc evitagen eht rof tnemtnio lartuen dna )C( puorg lortnoc( A). Daily wound observations were recorded for all groups of infectious specimens and granule tissue. Results: The average of non-growth in sensitive culture of staph was 3.19 mm and in resistant culture of staph was 6.17 mm. On 21st day, all scars of wounds in groups B and D were separated and the wound was free of infectious tissue, whereas groups A and C that the scars were clinging to the base. The findings were statistically significant. Mean wound in groups B, D, C, A was (95. 769 00 ± 86. 1835), (24. 204 97 ± 40. 204 6), (116.3620 ± 46.68171), (258. 21 93 ± 47. 635 0) mm respectively and the level of ulcer was significantly lower in ES intervention group and also the infectious tissue was not observed during treatment of group B and D wounds.Conclusion: MRSA susceptible and resistant of staph bacteria did not show resistance to excretory secretory substances of Lucilia sericata larvae. The debridement and recovery process was faster in the ES-treated group and the initial ointment than in the control group

secretion-excretion, Lucilia sericata, ointment, burn, wounds