Biochemical and computational study of an alginate lyase produced by Pseudomonas aeruginosa strain S21
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 23، شماره: 4
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 353
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شناسه ملی سند علمی:
JR_IJBMS-23-4_006
تاریخ نمایه سازی: 27 مرداد 1399
چکیده مقاله:
Objective(s): Alginates play a key role in mucoid Pseudomonas aeruginosa colonization, biofilm formation, and driving out of cationic antibiotics. P. aeruginosa alginate lyase (AlgL) is a periplasmic enzyme that is necessary for alginate synthesis and secretion. It also has a role in depolymerization of alginates. Using AlgLs in cystic fibrosis patients along with antibiotics enhances bacterial killing and host healing. In this study, we investigated the different biochemical properties of a newly isolated AlgL from P. aeruginosa S21 to complete the databank of AlgLsMaterials and Methods: The enzyme was extracted from the periplasmic space of the bacteria by the heat shock method. Using the TBA method, the enzyme activity and biochemical properties were assessed. The mutability of P. aeruginosa S21 AlgL to increase its thermal stability was investigated. The most favorable mutations were studied computationally. The molecular dynamics simulation (MDS) package GROMACS was used for determining the effect of S34R mutation on enzyme’s thermal stability.Results: Data showed that this enzyme has the best activity at 37 °C and pH 7.5 and it can degrade mannuronate blocks, guluronate blocks, and sodium alginate. After 7 hr at 80 °C, 45% of the enzyme activity was retained. This enzyme needed 15 min to completely degrade accessible sodium alginate. Tris buffer, pH 8.5 and Britton-Robinson buffer, pH 7.0 were the preferable buffers for the enzyme activity. MDS of native and mutated enzymes showed desirable results.Conclusion: P. aeruginosa S21 AlgL can be used in medical and industrial applications to degrade alginates.
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نویسندگان
Firoozeh Piroozmand
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Parinaz Ghadam
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Mahboobe Zarrabi
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Ahya Abdi Ali
Department of Microbiology, Faculty of Biological sciences, Alzahra University, Tehran, Iran
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