بررسی تهیه یک سیستم الیزای رقابتی به منظور تشخیص سرمی هپیودرموزیس بز

Goat warble Fly Infestation(GWFI),improperly named goat hypodermosis is a myiasis caused by larvae of przhevalskiana sp. Its economic impact is sever and in live animals,detection can be done by direct clinical examination of infested goat and palpation of larvae in the back or see the larvae under the skin (sub clermal region of back and flanks) of infested goat ofther slaughtered. Recently Serological methods used for immunodiagnonsis of hypodermosis in different animals.In the present study we describe a competitive ELISA for the detection of antibodies of przhevalskiana larvae in goat sera. Totally 32 sample sera from infested goat,42 sera from uninfected goat and 72 sera from filed sampling (random sampling from different parts of khoozestan province) were tested. Finaly, at cut off 2SD Sensitivity and specificity were 90% and 88.63%, and 90% and 88.66% in cut off 3SD.