Warton's jelly explant is a source of EGF and bFGF growth factors

Background: Warton's jelly is a source of mesenchymal stem cells and growth factors, for this reason Wharton's Jelly-Mesenchymal stem cells and release medium from explant can be considered as an appropriate source for regenerative medicine. Materials and methods: Warton's jelly explant were kept in DMEM low glucose for 10 days. After 4, 6, 8 and 10 days’ medium around explant were collected and kept in -20c°. The assays of growth factors were carried out with the use of Quntikine human EGF and bFGF ELISA commercial kits, together with SDS, polyacrylamide gel electrophoresis of tissue extracts. Growth factor such as EGF and bFGF were detected in Wharton's jelly. The amounts of these factors per gram of tissue vary from about 900 pg bFGF, to about 90 ng in EGF. The effect of this culture medium were analyzed on proliferation of HUVEC cells in compared with DMEM low glucose with MTT procedure for 1,3 and 5 days. Results: The results of this study showed that Warton's jelly explant culture medium could release EGF and bFGF into medium after 4 days. This medium could increase proliferation of HUVEC cells. Conclusion: Wharton's jelly explant culture medium after 4 days can be a suitable medium for cell culture. This method is easy and cost consuming for cell culture in regenerative medicine.