The Effect of Dracocephalum Kotschyi Alcoholic Extract on the BAX Expression in SKBR3 Cell Line

Introduction and Objectives: Breast cancer is considered as the most common disease threatening women around the world which is the fifth cause of death in Iran. Over the past few decades, derived compounds from plants have been a major source of anticancer clinical substances. Therefore, the effect of Dracocephalum Katschyi alcoholic extract on proliferation and BCL2 genes expression level on SKBR3 cell line in breast cancer is investigated in this study. Dracocephalum Kotschyi consists of apprximately one hundred different compounds such as Terpenoids, Flavonoids, and Alkaloids which are antioxidants. To achieve our goals different samples were prepared and were tested.Materials and Methods: One hundred grams of powder was produced from the Dracocephalum leaves and aerial parts were solved in 250 ml ethanol. The mix ratios were chosen to obtain samples having 10, 50, 100, and 500μg/ml concentrations. In addition, SKBR3 cell lines were cultured under 5% CO2 at 37 °C in DMEM. The effects of an alcoholic agent on cell line as well as BCL2 expression was investigated through exposing them for 24 hours. cDNA was synthesized from the extracted RNAs by a QuantiTed Reverse TranscriptionKit. Next, the BCL2 expression was evaluated.Results: An opposite correlation between the concentration of Dracocephalum Kotschyi and the cell proliferation suggested the anticancer characteristic of this plant. SKBR3 cells IC50 value in 24hrs was 102.1 and in 500μg/ml the most cell viability decrease was observed.Conclusions: The following conclusion can be drawn from the results of this investigation. Since Dracocephalum Katschyi alcoholic extract results in BCL2 expression decrease, it can be used in producing new anticancer medicines.