Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review

سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 403

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شناسه ملی سند علمی:

JR_IJP-16-1_001

تاریخ نمایه سازی: 26 آذر 1399

چکیده مقاله:

Molecular assays for detection of nucleic acids in biologic specimens are valuable diagnostic tools supporting clinical diagnoses and therapeutic decisions. Pre-analytical errors, which occur before or during processing of nucleic acid extraction, contribute a significant role in common errors which take place in molecular laboratories. Certain practices in specimen collection, transportation, and storage can affect the integrity of nucleic acids before analysis. Applying best practices in these steps, helps to minimize those errors and leads to better decisions in patient diagnosis and treatment. Widely acceptable recommendations, which are for optimal molecular assays associated with pre-analytic variables, are limited. In this article, we have reviewed most of the important issues in sample handling from bed to bench before starting molecular tests, which can be used in diagnostic as well as research laboratories. We have addressed the most important pre-analytical points in performing molecular analysis in fixed and unfixed solid tissues, whole blood, serum, plasma, as well as most of the body fluids including urine, fecal and bronchial samples, as well as prenatal diagnosis samples.

نویسندگان

Maryam Anvari

Molecular Pathology and Cytogenetics Division, Pathology Department, Children&#۰۳۹;s Medical Center, Tehran University of Medical Sciences, Tehran, Iran

Atoosa Gharib

Department of Pathology, Modarres Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Maryam Abolhasani

Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran; Hasheminejad Kidney Center, Iran University of Medical Sciences, Tehran, Iran

Aileen Azari-Yaam

Molecular Pathology and Cytogenetics Division, Pathology Department, Children&#۰۳۹;s Medical Center, Tehran University of Medical Sciences, Tehran, Iran

Farzaneh Gharalari

Department of Pathology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

Moeinadin Safavi

Molecular Pathology and Cytogenetics Division, Pathology Department, Children&#۰۳۹;s Medical Center, Tehran University of Medical Sciences, Tehran, Iran

Ali Zare-Mirzaie

Department of Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Mohammad Vasei

Cell-based Therapies Research Center, Digestive Disease Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

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