Calcium sensing receptor involving in therapy of embryonic stem cell transplantation alleviates acute myocardial infarction by inhibiting apoptosis and oxidative stress in rats

Hui Yuan; Guohong Yang; Shu Li; Li Li; Tao Wei; Gaochen Song; Hairong Luan; Jin Meng; Qi Wang; Yaquan Yu; Jian Sun

Calcium sensing receptor involving in therapy of embryonic stem cell transplantation alleviates acute myocardial infarction by inhibiting apoptosis and oxidative stress in rats

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 23، شماره: 10

سال انتشار: 1399

نوع سند: مقاله ژورنالی

زبان: انگلیسی

مشاهده: 196

فایل این مقاله در 7 صفحه با فرمت PDF قابل دریافت می باشد

دریافت فایل کامل مقاله

صدور گواهی نمایه سازی
من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

https://civilica.com/doc/1138527

شناسه ملی سند علمی:

JR_IJBMS-23-10_016

تاریخ نمایه سازی: 19 دی 1399

چکیده مقاله:

Objective(s): The aims of the present study were to investigate the expression of calcium sensing receptor (CaSR) at different times in acute myocardial infarction (AMI) rat myocardial tissue after mouse embryonic stem cells (mESCs) transplantation treatment and to assess its effects on apoptosis and oxidative stress of cardiomyocytes. Materials and Methods: The AMI rats were treated with mESCs, Calindol (a CaSR agonist) and Calhex231 (a CaSR inhibitor). Serum measurements, Echocardiographic analysis and TUNEL assay were performed. Myocardial ultrastructure changes were viewed by electron microscopy. Additionally, western blotting was used to detect the protein expressions.Results: Compared to the sham group, it was found that the expression levels of CaSR, caspase-3, cytoplasmic cytochrome C (cyt-C) and Bcl2-associated x (Bax), and the levels of Malondialdehyde (MDA) were significantly increased in both AMI and AMI + mESCs + Calindol groups with the development of myocardial infarction. Furthermore, the ultra-microstructure of cardiomyocyte was highly damaged, the expression levels of mitochondrial cyt-C and B-cell lymphoma 2 (Bcl-2) were significantly decreased, and there was decreased activity of superoxide dismutase (SOD). However, the combination of Calhex231 and mESCs transplantation could inhibit these changes. Conclusion: Our results suggested that CaSR expression in myocardial tissue of AMI rats was increased over time, and that Calhex231 could enhance the efficacy of ESCs transplantation for the treatment of AMI, which would be a new therapeutic strategy for the treatment of AMI.

کلیدواژه ها:

Acute myocardial infarction ، Apoptosis ، Calcium sensing receptor ، Cell transplantation ، Embryonic stem cell

نویسندگان

Hui Yuan