Investigation of enterobacter aerogenes effects on heavy oil from biological degradation aspects by GCxGC technique

Oil pollution affects the deterioration of the agrophysics soil characteristics, namely to the dysfunction of the water, air, thermal, oxidation-reduction and nutrient regimens. One of the methods to purify oil residue is bioremediation, in this way we investigate Enterobacter aerogenes effects on heavy oil by SARA, FT-IR, GC and GCxGC methods. The material required for the growth of E. aerogenes are carbon and phosphate. The E. aerogenes is injected with thioglycollate broth to crude oil which was distillated, and the samples were put for a month in an incubator. The results of this research illustrate that this bacterium has been able to survive in oil by isolating straight-run carbons with short and weak bounds from asphaltene and resin structures, as well as utilizing sulfur, nitrogen, and oxygen in the oil. In addition, it is found out this bacterium has had a positive effect on making light oil from heavy crude oil. Thereby, it is suitable for bioremediation in oil polluted sites and oil refinery for removing hydrocarbon pollutants from the environment.