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مقالات فارسیISIکنفرانسهاژورنالها

Bovine oocyte developmental competence and gene expression following co-culturing with ampullary cells: An experimental study

محل انتشار: مجله طب تولید مثل ایران، دوره: 19، شماره: 4
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 273

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استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1184550

شناسه ملی سند علمی:

JR_IJRM-19-4_007

تاریخ نمایه سازی: 1 اردیبهشت 1400

چکیده مقاله:

Background: There is no sufficient information on the impact of bovine ampullary oviductal epithelial cells (BAOECs) on in vitro oocyte maturation competence and gene expression. Objective: This study aimed to examine the oocyte developmental competence following co-culturing with a monolayer of fresh and frozen-thawed ampullary cells. Materials and Methods: Bovine cumulus-oocyte complexes (COCs) were distributed into three groups: control group; where in COCs were cultured in cell-free media for 24 hr and FML and FTML groups in which the COCs were cultured in maturation media for 18 hr and then transferred into a media containing fresh and frozen-thawed BAOECs monolayer, respectively (BAOECs were extracted from the oviducts of slaughtered cattle and were then cultured freshly or frozen-thawed) for a further 6 hr. After 24 hr, the expanded COCs were evaluated for nuclear maturation, fertilization rate, and gene expression (GDF9, StAR, CASP3, and FSHr). Results: Nuclear maturation rate in the FTML group was significantly higher than the control group (p = 0.02). The fertilization rate of FTML group was significantly higher than the control and FML groups (p = 0.05 and p = 0.03, respectively). In terms of gene expression, GDF9 were upregulated in the presence of the BAOECs during the last 6 hr of the in vitro maturation (p < 0.001). Furthermore, the expression of the StAR gene in the FTML group was higher than the other groups (p = 0.02). Conclusion: Ampullary cells co-culturing (especially frozen-thawed cells) for in vitro maturation of bovine oocytes yields encourages the results and demonstrates the beneficial effect of co-culture on gene expression and developmental competence.  

کلیدواژه ها:

Ampulla ، Bovine ، Fertilization ، Gene expression ، IVM. ، بلوغ تخمک ، بیان ژن ، سلول­های اپیتلیال آمپولا ، لقاح.

نویسندگان

Mehdi Azari

Department of Anatomical Sciences, School of Medicine, AJA University of Medical Sciences, Tehran, Iran. Department of Animal Reproduction, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Mojtaba Kafi

Department of Animal Reproduction, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Anise Asaadi

Department of Animal Reproduction, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Zohreh Pakniat

Department of Animal Reproduction, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Beheshteh Abouhamzeh

Department of Anatomical Sciences, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.

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