The Effect of Eight Weeks of Intense Aerobic Exercise on Vaspin and Chemerin Plasma Changes in Female Spraw Dawly Rats

Background and Objective: The adipokines or adipocytokines are cytokines secreted from adipocyte tissue including Vaspin and chemerin. The purpose of the present study was to investigate the effect of eight weeks of intense aerobic exercise on vaspin and chemerin level changes in female Spraw dawly rats. Materials and Methods: Among the Spraw Dawly rats available in the laboratory of Shiraz University of Medical Sciences, ۳۵ samples were selected and divided into control (۱۵ rats) and experimental (۲۰ rats) groups. The necessary controls were conducted in terms of nutrition, sex (female) and age (two months old). For ۸ weeks, the training group rats performed a high-intensity exercise in accordance with the training program, consisting of running on a treadmill ۵ times a week. This exercise was carried out progressively and in compliance with the principle of overload. The data was analyzed using independent t-test by SPSS (version ۱۶). In order to determine the relationship between the study variables, Pearsonchr('۳۹')s correlation coefficient was utilized. Results: The research findings suggest that physical exercise reduces vaspin protein levels (P<۰.۰۰۰۱) and increases serum levels in chemerin (P<۰.۰۰۰۱). Conclusion: It was found out that the response of adipokines in female rats to intense exercises (for ۸ weeks) is different though, it cannot be claimed that all of them exhibit increasing or decreasing effects. The response is probably influenced by the duration or the intensity of the exercise. References ۱- Hida K, Wada J, Eguchi J, et al. Visceral adipose tissue-derived serine protease inhibitor: a unique insulin-sensitizing adipocytokine in obesity. Proc Natl Acad Sci USA. ۲۰۰۵ ۱۰۲: ۱۰۶۱۰-۵. ۲- Hashemi F, Yaghmaei P, Saadati N, et al. Association of serum adipsin levels with polycystic ovarian syndrome. Razi J Med Sci. ۲۰۱۲ ۱۹: ۱-۶۰. ۳- Wada J. Vaspin: a novel serpin with insulin-sensitizing effects. Expert Opin. Investig Drugs. ۲۰۰۸ ۱۷: ۳۲۷-۳۳. ۴- Brunetti L, Di Nisio C, Recinella L, et al. Effects of vaspin, chemerin and omentin-۱ on feeding behavior and hypothalamic peptide gene expression in the rat. Peptides. ۲۰۰۱ ۳۲: ۱۸۶۶-۷۱. ۵- Tan BL, Heutling D, Chen J, et al. Metformin decreases the adipokine vaspin in overweight women with polycystic ovary syndrome concomitant with improvement in insulin sensitivity and a decrease in insulin resistance. Diabetes. ۲۰۰۸ ۵۷: ۱۵۰۱-۱۵۰۷. ۶- Gulcelik NE, Karakaya J, Gedik A, Usman A, Gurlek A. Serum vaspin levels in type ۲ diabetic women in relation to microvascular complications. Eur J Endocrinol. ۲۰۰۹ ۱۶۰: ۶۵-۷۰. ۷- Bozaoglu K,Bolton K, McMillan J, et al. Chemerin is a novel adipokine associated with obesity and metabolic syndrome. Endocrinology. ۲۰۰۷ ۱۴۸: ۴۶۸۷-۹۴. ۸- Roh SG, Song SH, Choi KC, et al. Chemerin: a new adipokine that modulates adipogenesis via its own receptor. Biochem Biophys Res Commun. ۲۰۰۷ ۳۶۲: ۱۰۱۳-۸. ۹- Ernst MC, Sinal CJ. Chemerin: at the crossroads of inflammation and obesity. Trends Endocrinol Metab. ۲۰۱۰ ۲۱: ۶۶۰-۷. ۱۰- Keshtkasr B, Daryanoosh F, Nabizade F, Tanideh N, Salesi M. The effect of traning program with moderate and high intensities on npy hormone and ghrelin in fat asprague- dawley rats. J Zanjan Univ Med Sci. ۲۰۱۴ ۲۲: ۹۶-۱۱۰. ۱۱- Safarzade A, Garakani TA. Effects of progressive resistance training on serum levels of vaspin and some inflammatory markers in male rats. Koomesh. ۲۰۱۲ ۱۴: ۹۷-۱۰۳. ۱۲- Youn BS, Klöting N, Kratzsch J, et al. Serum vaspin concentrations in human obesity and type ۲ diabetes. Diabetes. ۲۰۰۸ ۵۷: ۳۷۲-۷۷. ۱۳- Kim JY, Kim ES, Jeon JY, Jekal Y. Improved insulin resistance, adiponectin and liver enzymes without change in plasma vaspin level after ۱۲ weeks of exercise training among obese male adolescents. Korean J Obes. ۲۰۱۱ ۲۰: ۱۳۸-۴۶. ۱۴- Chakaroun R, Raschpichler M, Klöting N, et al. Effects of weight loss and exercise on chemerin serum concentrations and adipose tissue expression in human obesity. Metab Clin Exper. ۲۰۱۲ ۶۱: ۷۰۶-۱۴. ۱۵- Chen JX, Yang WY, Liang R, Lu DY, Zhou WA. Experimental study of the effects of long term and high intensity exercise on plasma ß- endorphin, dynorphin A۱-۱۳, leucine- enkephalin and lactate in rats. Chin J Appl Physiol. ۱۹۹۷ ۱۴: ۳۷۱-۸. ۱۶- Safarzade A, Gharakhanlou R, Hedayati M, Talebi-Garakani E. The effect of ۴ weeks resistance training on serum vaspin, Il-۶, CRP and TNF-Α concentrations in diabetic rats. Iran J Endoc Metab. ۲۰۱۱ ۱۴: ۶۸-۷۴. ۱۷- Oberbach A, Kirsch K, Lehmann S, et al. Serum vaspin concentrations are decreased after exercise-induced oxidative stress. Obes Facts. ۲۰۱۰ ۳: ۳۲۸-۳۱. ۱۸- Lee JA, Park HS, Song YS, et al. Relationship between vaspin gene expression and abdominal fat distribution of Korean women. Endocr J. ۲۰۱۱ ۵۸: ۶۳۹-۴۶. ۱۹- Cho JK, Han TK, Kang HS. Combined effects of body mass index and cardio/respiratory fitness on serum vaspin concentrations in Korean young men. Eur J Appl Physiol. ۲۰۱۰ ۱۰۸: ۳۴۷-۵۳. ۲۰- Wittamer V, Franssen J.D, Vulcano M, et al. Specific recruitment of antigen-presenting cells by chemerin, a novel processed ligand from human inflammatory fluids. J Exp Me. ۲۰۰۳ ۱۹۸: ۹۷۷-۸۵. ۲۱- Roman A, Parlee S, Sinal C. Chemerin: a potential endocrine link between obesity and type ۲ diabetes. Endocrine. ۲۰۱۲ ۴۲: ۲۴۳-۵۱. ۲۲- Hart R, Greaves DR. Chemerin contributes to inflammation by promoting macrophage adhesion to VCAM-۱ and fibronectin through clustering of VLA-۴ and VLA-۵. J Immunol. ۲۰۱۰ ۱۸۵: ۳۷۲۸-۳۹. ۲۳- Saremi A, Shavandi N, Parastesh M, Daneshmand H. Twelve-week aerobic training decreases chemerin level and improves cardiometabolic risk factors in overweight and obese men. Asian J Sport Med. ۲۰۱۰ ۱: ۱۵۱-۸.