Diabetes mellitus can cause cardiomyopathy disorders by inducing the aging pathway
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 24، شماره: 5
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 267
فایل این مقاله در 5 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJBMS-24-5_010
تاریخ نمایه سازی: 18 اردیبهشت 1400
چکیده مقاله:
Objective(s): In this study, cardiovascular disorders were examined with a focus on the aging pathway and autophagy involvement in cardiac samples isolated from male rats with type ۲ diabetes mellitus.Materials and Methods: In the present study, male Wistar rats became diabetic with the help of a high-fat diet. Gene and protein expression levels (to evaluate Tumor Necrosis Factor-α, TNF-α) were measured by the ELISA method. Nrf۲, p۳۸, and GSK-۳β proteins in cardiac tissue samples were measured by the western blotting method. Autophagy examination was performed with immunofluorescence staining. Finally, quantitative results were calculated using statistical analysis.Results: The expression of beta-galactosidase genes had a significant increase in the diabetic group (P=۰.۰۰۰۱). However, there was no significant difference in the expression of the SERCA۲a gene between the diabetic and control groups. In terms of protein expression, the amount of TNF-α protein in the diabetic group was significantly different from that of the control group (P=۰.۰۱۰۲). The expression levels of p۳۸, Nrf۲, and GSK-۳β proteins increased compared with the control group. The use of the LC۳ immunofluorescence staining technique revealed that autophagy increased in the diabetic group.Conclusion: Type ۲ diabetes mellitus in rats will increase aging in cardiac cells. Examination of the signaling pathway indicates that this effect is related to the increase of ROS and the activity of the signaling pathway. In response, the cardiac cells try to maintain their homeostasis by increasing autophagy and decreasing inflammatory cytokines.
کلیدواژه ها:
نویسندگان
Mahdi Ahmadi
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Shirin Saberianpour
Vascular and Endovascular surgery research center, Mashhad Medical university,Mashhad,Iran
Morteza Heidarzadeh
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Jafar Rezaie
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :