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Evaluation of survival rate using liposome containing soluble antigens (SA) against Toxoplasma gondii infection in BALB/c mice

محل انتشار: مجله علوم نانو، دوره: 8، شماره: 3
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 266

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لینک ثابت به این مقاله:
https://civilica.com/doc/1240587

شناسه ملی سند علمی:

JR_NAMJ-8-3_003

تاریخ نمایه سازی: 19 تیر 1400

چکیده مقاله:

Objective(s): Toxoplasma gondii, an obligate intracellular protozoan parasite, is widespread across the world. It causes congenital disease and abortion in humans and domestic animals. One of the major concerns in parasitology, thus, is an effective vaccine development to control Toxoplasmosis. Materials and Methods: In the present research, a nano-liposomal vaccine containing soluble antigens (SA) was designed to evaluate the immunity and protective efficacy against T. gondii infection in BALB/c mice. Soluble antigens (SA) were achieved from tachyzoites, encapsulated in the liposome, and investigated via scanning electron microscope. Three times with ۲-week intervals, BALB/c mice were immunized subcutaneously with different formulations. The level of protection against infection was assessed through the percent survival survey of BALB/c mice after challenge with tachyzoites of T. gondii RH strain; also, the type of generated immune response was determined by evaluating the generation of cytokine (IFN-γ, IL-۴) and titration of IgG isotypes.Results: The immunization with liposome DSPC+ SA and liposome DSPC+ Imiquimod + SA induced a substantial increase in anti-Toxoplasma IgG antibody as compared to the PBS group (p <۰.۰۵). The IgG۲a and IFN-γ secretion highest levels were seen with liposome DSPC+ Imiquimod + SA more than the control group (p <۰.۰۱) and (p <۰.۰۰۰۱), respectively. After challenge with tachyzoites, less mortality was detected in the immunized mice by liposome DSPC + Imiquimod + SA that was meaningfully different (p <۰.۰۱) in comparison to other groups.Conclusion: Vaccination with liposome DSPC + Imiquimod + SA showed more survival rate and cellular immune reaction against T. gondii.

کلیدواژه ها:

Cationic Liposome ، Imiquimod ، Immune response ، Toxoplasma gondii

نویسندگان

Mahdi Kavand

Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran

Ahmad Mehravaran

Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran

Elham Pahlavani

Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran

Hadi Mirahmadi

Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran

Javad Akhtari

Toxoplasmosis Research Center, Department of Medical Nanotechnology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Mansour Rahmati-Balaghaleh

Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran

Soudabeh Etemadi

Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran

Leili Mohammadi

Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran

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