Evaluation of Antibacterial and Cytotoxic Effects of K۴ Synthetic Peptide
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 345
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شناسه ملی سند علمی:
JR_TUMS-3-2_005
تاریخ نمایه سازی: 16 مرداد 1400
چکیده مقاله:
Introduction: With increasing antimicrobial resistance to common antibiotics, development of alternative therapeutic strategies is necessary. Also, antibacterial peptide with numerous behavior and different properties such as net charge, hydrophobicity, length, etc. could act against pathogenic microorganism. In recent years, novel peptides with activity against wide range of bacteria have been introduced.Methods: In this study, seventeen pathogenic bacteria were chosen to study the antibacterial effect of K۴ peptide using MIC and MBC assays. The therapeutic index (TI) of this peptide experimentally calculated by the ratio of HC۵۰ to MIC as a parameter to represent the specificity of AMP. In silico analysis was performed to predict the physico-chemical properties, structures, and behavior of this peptide. In vitro cytotoxic effect of peptide was evaluated on the Hela cell line using MTT assay, and the amount of macrophage nitric oxide production was measured by Griess method on the J۷۷۴ cell line. Results: Peptide concentrations of ۲۵-۴۰۰ µg/ml was seen as the MIC value results for different bacteria. MBC assay showed such a result with concentration of more than ۲۵-۴۰۰ µg/ml. The result of hemolysis assay was ۲۴ percent at ۱ mg/ml concentration. The amount of nitric oxide production of macrophage cell line J۷۷۴ was ۲۵.۹۸۷۳ µM at ۶.۳ µg/ml peptide concentration. Conclusions: K۴ peptide had strong antibacterial effect on some bacteria such as B. melitensis. This peptide may have a role in immunity with nitric oxide production. Additionally, it enhances bacterial killing mechanism of macrophage, making this peptide a potential agent against pathogens.
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نویسندگان
Kazem Ahmadi
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Mahdi Fasihi Ramandi
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
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