Identification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion

سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 160

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شناسه ملی سند علمی:

JR_JHPR-4-14_003

تاریخ نمایه سازی: 7 شهریور 1400

چکیده مقاله:

Purpose: Modern onion breeding is almost completely based on the production of hybrid seed. This project was performed to evaluate the effectiveness of marker-assisted selection (MAS) in identification of the cytoplasmic types and Ms locus in ۱۲۳ onion accessions. Research method: Three cytoplasmic markers cob, accD and MK were used to identify the sterility (S) from the fertility (N) cytoplasm and four nuclear molecular markers (OPT, PsaO, Jnurf-۱۳ and AcSKP۱) were used for genotyping of Ms alleles. Findings: The results showed that the two accD and cob markers were quite similar in the detection of the type of cytoplasm with ۱۰۰% male sterility for male sterile lines and ۱۰۰% fertility for maintainer lines. The MK marker was able to distinguish T-type cytoplasm as well. Based on the results, the frequency of fertility (N) was much more than the frequency of sterility (S and T) cytoplasm found to be ۹۰% in Dorche (pop.۱), ۱۰۰% in Dorche (pop.۲) and Kashan based on marker cob and accD and with MK marker, was found to be ۸۰%, ۹۰% and ۸۲% in Dorche (pop.۱), Dorche (pop.۲) and Kashan, respectively. Limitations: In this study, nuclear markers were not successful due to not finding linkage disequilibrium with the Ms locus, suggested more markers to be evaluated. Originality/Value: Molecuar markers were very suitable for the indentification of S or N lines. Cytotype (N/S) determination of plants by usingmolecular markers (cob, accD and MK), could easily reduce the population size required for the production of onion hybrid seeds.

نویسندگان

Rahele Dehghani

Department of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran, ۸۴۱۵۶۸۳۱۱۱

Zahra Abbasi

Horticulture Crops Research Department, Isfahan Agricultural and Natural Resources Research and Education Center, (AREEO), Isfahan, Iran

Majid Talebi

Department of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran, ۸۴۱۵۶۸۳۱۱۱

Emad Shahmansouri

Horticulture Crops Research Department, Isfahan Agricultural and Natural Resources Research and Education Center, (AREEO), Isfahan, Iran

Badrodin Ebrahim Sayed Tabatabaei

Department of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran, ۸۴۱۵۶۸۳۱۱۱

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