Genotoxic Effects of Heavy Metals on Mitotic Chromosomes of Trigonella Foenum-Graecum L.
محل انتشار: مجله منابع ژنتیک، دوره: 7، شماره: 2
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 201
فایل این مقاله در 7 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_SGR-7-2_012
تاریخ نمایه سازی: 10 شهریور 1400
چکیده مقاله:
Lead, cadmium, and copper are common environmental pollutants in most industrialized countries. Soil with heavy metal pollution has raised concern in recent years due to its possible destructive effects on plants system. In the present investigation, we studied the genotoxic effect of lead, cadmium, and copper on mitotic chromosomes of Trigonella foenum-graecum L. for the first time. The root tips of T. foenum-graecum were treated with four graded concentrations (viz. ۵۰, ۱۰۰, ۱۵۰, and ۲۰۰ ppm) of lead, cadmium, and copper. After hydrolyzing the root end in hydrochloric acid solution, they were stained with acetocarmine. After squashing the root end, they were studied under a microscope. To this end, the mitotic index (MI) and the total percentage of abnormality (TAP%) were analyzed. Studying of the root end of T. foenum-graecum showed that this plant is diploid and its chromosomal base number is ۲x=۲n=۱۶. Cytological monitoring revealed that Pd, Cd, and Cu exhibit mitodepressive behaviors at higher concentrations. Moreover, the mitotic index decreases, but the incidence of different anomalies, such as sticky chromosome, c-mitosis, micronucleus, laggard chromosome, bridge, and precocious movement, increase through increasing heavy metal concentration. Among the mitotic abnormalities observed in all treatments, the highest percentage was related to the sticky chromosome. The highest genotoxic potential was observed in the lead, followed by cadmium and copper. The results showed that heavy metal pollution led to a significant MI reduction and TAP% increase in root tip meristems of T. foenum-graecum. This should be considered as a risk warning of the environmental pollution on plants, especially medicinal plants.
کلیدواژه ها:
نویسندگان
Fatemeh Hajmoradi
Department of Biology, Payame-Noor University, Tehran, Iran
Mehdi Kakaei
Department of Agriculture, Payame-Noor University, Tehran, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :