Quantification of Pla or ۳, a Platanus orientalis Allergen, Grown under Different Environmental Conditions, by Sandwich ELISA
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 188
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شناسه ملی سند علمی:
JR_RBMB-5-1_006
تاریخ نمایه سازی: 10 شهریور 1400
چکیده مقاله:
Background: Platanus species are widely cultured around the world and considered an important cause of allergic reactions. In the present study, we developed a sandwich ELISA to quantify Pla or ۳ allergen in P. orientalis pollen extracts grown near high-traffic roads and compared it to pollen extracts collected from rural areas as control.
Methods: Pollen samples were collected from three polluted and two unpolluted sites in Mashhad, northeast Iran. Recombinant Pla or ۳ was expressed and used for polyclonal antibody production in rabbit. A sandwich ELISA was developed and validated to quantify Pla or ۳ levels in pollen extracts from the different sites.
Results: The coefficients of variation (CVs) for the intra- and inter- day assays were less than ۵ and ۱۸%, respectively. The working range of the standard curve was between ۰.۱ and ۲۵ ng/ml, with the detection limit being ۰.۰۳۷ ng/ml. The recovery percentage was ۸۸-۱۰۶.۴% at working concentrations from ۰.۳۱ to ۲۶.۵ ng/ml. Pla or ۳ levels were significantly greater in pollens grown near high-traffic roads than in those grown in rural regions (p < ۰.۰۰۰۱).
Conclusions: A sandwich ELISA was developed and validated to quantify Pla or ۳ in pollen extracts. Using this validated ELISA, we showed a substantial difference between the amounts of Pla or ۳ in pollens grown in different environments. This finding should be considered in developing public policies to reduce traffic pollution, which leads to reduced allergic reactions in atopic subjects.
کلیدواژه ها:
نویسندگان
Farnaz Sedghy
Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran
Mojtaba Sankian
Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran
Maliheh Moghadam
Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran
Abdol-Reza Varasteh
Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran