Construction of a Novel DNA Vaccine Candidate Encoding an HspX-PPE۴۴-EsxV Fusion Antigen of Mycobacterium tuberculosis
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 141
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شناسه ملی سند علمی:
JR_RBMB-4-2_005
تاریخ نمایه سازی: 10 شهریور 1400
چکیده مقاله:
Background: Mycobacterium tuberculosis is the causative agent of tuberculosis (TB). Bacille Calmette-Guerin (BCG) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult TB vaccine. The aim of this study was to develop a new tuberculosis DNA vaccine candidate encoding a recombinant HspX-PPE۴۴-EsxV fusion antigen of M. tuberculosis.
Methods: A fusion DNA segment consisting of HspX, linker, PPE۴۴, linker, and EsxV, after codon optimization, was designed. The fusion DNA was cloned and its sequence confirmed. Then, expression of a recombinant pcDNA۳.۱ (+)/HspX-PPE۴۴-EsxV plasmid in Chinese hamster ovary (CHO) cells was verified by RT-PCR and Western-blot analysis.
Results: A ۱۹۶۸ bp band in RT-PCR and a ۶۸ kDa band on Western-blot analysis confirmed transcription and expression of recombinant hspX-ppe۴۴-esxV in eukaryotic cells.
Conclusions: A recombinant DNA segment encoding the HspX-PPE۴۴-EsxV fusion antigen of M. tuberculosis was constructed and considered to be tested as a new TB DNA vaccine candidate.
کلیدواژه ها:
نویسندگان
Bagher Moradi
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Mojtaba Sankian
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Yousef Amini
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Zahra Meshkat
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran