Investigation of DNA Integration into Reproductive Organs Following Intramuscular Injection of DNA in Mice
سال انتشار: 1391
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 198
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شناسه ملی سند علمی:
JR_RBMB-1-1_004
تاریخ نمایه سازی: 10 شهریور 1400
چکیده مقاله:
Background: DNA immunization with plasmid DNA encoding bacterial, viral, parasitic, and tumor antigens has been reported to trigger protective immunity. The use of plasmid DNA vaccinations against many diseases has produced promising results in animal and human clinical trials; however, safety concerns about the use of DNA vaccines exist, such as the possibility of integration into the host genome, and elicitation of adverse immune responses.
Methods: In this study, we examined the potential integration and bio-distribution of pcDNA۳.۱+PA, a new vaccine candidate with GenBank accession # EF۵۵۰۲۰۸, encoding the PA۶۳ gene, in reproductive organs of mice; ovaries and uterus in female, and testis in male. Animals of both sexes were injected intramuscularly with pcDNA۳.۱+PA. Host genome integration and tissue distribution were examined using PCR and RT-PCR two times monthly for six months.
Results: RT-PCR confirmed that pcDNA۳.۱+PA was not integrated into the host genome and did not enter reproductive organs.
Conclusions: This finding has important implications for the use of pcDNA۳.۱+PA plasmid as a vaccine and opens new perspectives in the DNA vaccine area.
کلیدواژه ها:
نویسندگان
Fatemeh Vahedi
Razi Vaccine & Serum Research Institute, Mashhad, Iran
Najmeh Nazari
Biochemistry Department, Payam-e-Noor University, Mashhad, Iran
Shirin Arbabi
Biochemistry Department, Payam-e-Noor University, Mashhad, Iran
Yasser Peymanfar
Razi Vaccine & Serum Research Institute, Mashhad, Iran