Chronoefficacy of alpha-lipoic acid in combination with nifedipine and glimepiride attenuates testicular toxicity in diabetic rats
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 97
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شناسه ملی سند علمی:
JR_PJMT-10-3_001
تاریخ نمایه سازی: 11 مهر 1400
چکیده مقاله:
Background: Knowledge of chronopharmacology and disease rhythms may provide additional therapeutic options for diabeticcomplications. The present research investigates the effect of chronomodulated alpha-lipoic acid/ nifedipine/ glimepiride combinationin oxidative stress-mediated testicular toxicity in diabetic rats. A total of seven rat groups were used for the following study.Methods: A group of non-diabetic rats and a group of diabetic rats were treated with ۱ mL/kg of water to serve as normal and diabeticcontrols respectively. All other groups were diabetic and received ۱۰ mg/kg glimepiride at ۲۰:۰۰h. Additionally, groups four to sevenwere treated with ۲۰ mg/kg nifedipine at ۰۸:۰۰h while groups five to seven received additional treatments with alpha-lipoic acid(ALA) at ۰۸:۰۰h, ۱۴:۰۰h and ۲۰:۰۰h respectively. Rats were euthanized after four weeks of oral treatment and the epididymis andtestis were excised for assessment of fertility markers. Serum testosterone and relative testes weights were measured. The right testeswere preserved in phosphate buffer for cholesterol and antioxidant assay while the left testes were fixed in formalin for histologicalstudies.Results: All rat groups treated with ALA showed significantly (p ≤ ۰.۰۱) better prognostic values for all markers assessed comparedto the diabetic control group. Those treated with ALA at ۲۰:۰۰h showed better prognosis (p ≤ ۰.۰۵) than treatment at other time points,showing values similar to the normal.Conclusion: Time-dependent triple therapy with alpha-lipoic acid, nifedipine and glimepiride mitigates oxidative stress-mediatedtesticular injury in diabetic rats and its clinical benefits may be explored using equivalent circadian timing in men.
کلیدواژه ها:
نویسندگان
Michael Oraebosi
department of pharmacology and therapeutics ABU zaria
Temidayo Olurishe
department of pharmacology and therapeutics ABU zaria
Sherifat Anafi
department of pharmacology and therapeutics ABU zaria
Mohammed Bisalla
Department of Veterinary Pathology and Microbiology, Ahmadu Bello University, Zaria, Nigeria
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