In vitro assessment of alendronate toxic and apoptotic effects on human dental pulp stem cells
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 21، شماره: 9
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 140
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شناسه ملی سند علمی:
JR_IJBMS-21-9_006
تاریخ نمایه سازی: 27 مهر 1400
چکیده مقاله:
Objective(s): Osteonecrosis of the jaw, as an exposed necrotic bone in the oral cavity, is one of the adverse effects of bisphosphonates, which have an affinity for bone minerals. This study investigates the cytotoxic effects of alendronate (ALN) as a nitrogen-containing bisphosphonate, on human dental pulp stem cells (hDPSCs). Materials and Methods: The mesenchymal stem cells (MSCs), obtained from third molar tooth pulps were characterized by immunophenotyping assay in order to identify surface markers to evaluate their expression. To detect multipotency hDPSCs, they were differentiate into osteocytes and adipocytes. Cell proliferation was measured by MTT assay. PI staining of DNA fragmentation by flowcytometry (sub-G۱ peak) was performed for determination of apoptotic cells and Bax, Bcl-۲, and cleaved caspase ۳ expressions. Protein expression was detected by Western blotting. Results: As the results revealed, ALN decreased viable cells (in ۰.۸–۱۰۰ µM) after ۷۲ hr and ۱۶۸ hr (P<۰.۰۰۱), significantly. ALN could lower cell proliferation in hDPSCs in a concentration and time-dependent manner. Sub-G۱ peak as an indicator of flowcytometry histogram of treated cells by ALN, showed apoptosis was involved in ALN-induced cytotoxicity. Expressions of cleaved caspase ۳ and Bax protein, as pro-apoptotic proteins, were increased and Bcl-۲ protein as anti-apoptotic protein was decreased in response to increases in the concentration of ALN (۰.۸–۲۵ µM).Conclusion: Long-term effects of ALN on cell proliferation and apoptosis in hDPSCs can result in either initiation or potentiation of ALN-induced osteonecrosis.
کلیدواژه ها:
نویسندگان
Solmaz Pourgonabadi
Department of Oral and Maxillofacial Surgery, Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran
Ahmad Ghorbani
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
zahra Tayarani najaran
Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Seyed Hadi Mousavi
Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
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