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مقالات فارسیISIکنفرانسهاژورنالها

Mangosteen peel extract (Garcinia mangostana L.) as protective agent in glucose-induced mesangial cell as in vitro model of diabetic glomerulosclerosis

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 21، شماره: 9
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 153

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استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1295238

شناسه ملی سند علمی:

JR_IJBMS-21-9_015

تاریخ نمایه سازی: 27 مهر 1400

چکیده مقاله:

Objective(s): This study aims to evaluate the activity of mangosteen peels extract (MPE) as protection agent on induced-glucose mesangial cells (SV۴۰ MES ۱۳ cell line (Glomerular Mesangial Kidney, Mus Musculus)).  Materials and Methods: MPE was performed based on maceration method. Cytotoxic assay was performed based on MTS (۳-(۴,۵-dimethylthiazol-۲-yl)-۵-(۳-carboxymethoxyphenyl)-۲-(۴-sulfophenyl)-۲H-tetrazolium) method, while the level of TGF-β۱ (Transforming growth factor-β۱) and fibronectin in glucose-induced mesangial cells were assayed and determined using ELISA KIT. Results: In viability assay, MPE ۵ and ۲۰ µg/ml has the highest activity to increase cells proliferation in glucose-induced mesangial cells at ۵, ۱۰, and ۱۵ days of incubation in glucose concentration (۵ and ۲۵ mM) (P<۰.۰۵). In inhibitory activity of TGF-β۱ and fibronectin level, MPE ۵ µg/ml (glucose-induced ۵ mM) show the lowest level compared to positive control and other treatments (P<۰.۰۵).Conclusion: MPE can increase cell proliferation in glucose-induced mesangial cells and significantly reduce the level of TGF-β۱ and fibronectin. MPE activity has correlates to inhibit the diabetic glomerulosclerosis condition and may increase mesangial cell proliferation.

کلیدواژه ها:

Fibronectin ، Glomerulosclerosis ، Garcinia mangostana ، Mesangial cell ، Transforming growth factor-β۱

نویسندگان

Wahyu Widowati

Faculty of Medicine, Maranatha Christian University, Bandung ۴۰۱۶۴, West Java, Indonesia

Dian Laksmitawati

Faculty of Pharmacy, Pancasila University, Jakarta Selatan ۱۲۶۳۰, DKI Jakarta, Indonesia

Teresa Wargasetia

Faculty of Medicine, Maranatha Christian University, Bandung ۴۰۱۶۴, West Java, Indonesia

Ervi Afifah

Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung ۴۰۱۶۳, West Java, Indonesia

Annisa Amalia

Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung ۴۰۱۶۳, West Java, Indonesia

Yukko Arinta

Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung ۴۰۱۶۳, West Java, Indonesia

Rizal Rizal

Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung ۴۰۱۶۳, West Java, Indonesia

Tri Suciati

School of Pharmacy Bandung Insitute of Technology, Bandung ۴۰۱۳۲, West Java, Indonesia

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