Histomorphometric evaluation of mice testicular tissue following short- and long-term effects of lipopolysaccharide-induced endotoxemia
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 21، شماره: 1
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 122
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شناسه ملی سند علمی:
JR_IJBMS-21-1_008
تاریخ نمایه سازی: 27 مهر 1400
چکیده مقاله:
Objective(s):Lipopolysaccharide (LPS)-induced endotoxemia is known to cause male infertility. This study was designed to explore the effects of bacterial LPS on histomorphometric changes of mice testicular tissues. Materials and Methods: In experiment ۱, a pilot dose responsive study was performed with mice that were divided into five groups, receiving ۳۶۰۰۰, ۱۸۰۰۰, ۹۰۰۰, and ۶۷۵۰ µg/kg body weight (B.W) of LPS or only saline (control). White blood cells (WBC) were observed for ۳ days after LPS inoculation. In experiment ۲, two groups of mice were treated with ۶۷۵۰ µg/kg B.W of LPS or only saline (control). Five cases from each experimental group were sacrificed at ۳, ۳۰, and ۶۰ days after LPS inoculation. Left testes were fixed in Bouin’s solution, and stained for morphometrical assays. Results: Time-course changes of WBC obtained from different doses of LPS-treated mice showed that inoculation of ۶۷۵۰ µg/kg B.W produced a reversible endotoxemia that lasts for ۷۲ hr and so it was used in the second experiment. In experiment ۲, during the first ۳ days, no significant changes were observed in the evaluated parameters instead of seminiferous tubules diameter. Spermatogenesis, Johnsen’s score, meiotic index, and epithelial height were significantly affected at ۳۰th day. However, complete recovery was only observed for the spermatogenesis at day ۶۰. Interestingly, deleterious effects of LPS on spermatogonia were only seen at ۶۰th day (P<۰.۰۵). Conclusion: Endotoxemia induced by LPS has long-term detrimental effects on spermatogonia and later stage germ cells, which are reversible at the next spermatogenic cycle.
کلیدواژه ها:
نویسندگان
Oveis Jafari
Graduate Student of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Homayoon Babaei
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Reza Kheirandish
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Amir-Saied Samimi
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Ali Zahmatkesh
Graduate Student of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
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