Anti-hyperglycemic and antioxidant potential of Croton bonplandianus. Bail fractions in correlation with polyphenol content

Uma Dharshini Karuppiah Vijayamuthuramalingam; Rajeswari Rajaram; Kalaivani Madhavaram Kuppusamy; Bhavana Jonnalagadda; Sumathy Arokiasamy

Anti-hyperglycemic and antioxidant potential of Croton bonplandianus. Bail fractions in correlation with polyphenol content

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 20، شماره: 12

سال انتشار: 1396

نوع سند: مقاله ژورنالی

زبان: انگلیسی

مشاهده: 102

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لینک ثابت به این مقاله:

https://civilica.com/doc/1295627

شناسه ملی سند علمی:

JR_IJBMS-20-12_015

تاریخ نمایه سازی: 28 مهر 1400

چکیده مقاله:

Objective(s):Diabetes mellitus, a carbohydrate metabolic disorder, occurs due to absolute or relative deficiency of insulin. Current treatment strategies involve either preventing or delaying the intestinal absorption of glucose to lower the levels of postprandial hyperglycemia (PPHG). Herbal remedies have been since ancient times for treating diabetes mellitus. Therefore, identifying novel phytocompounds with α-amylase and α-glucosidase inhibitory activity that would reduce the glucose absorption as well as the rise in postprandial blood glucose level is vital. Consequently, the present study was aimed to investigate the anti-hyperglycemic activity of Croton bonplandianusagainst these pancreatic enzymes. Materials and Methods: The methanol extract of C. bonplandianusleaf was prepared and further fractionation was performed with n-hexane, ethyl acetate and chloroform. The antioxidant activity and anti-hyperglycemic activity of the extracts and its fractions were determined. Further, GC-MS analysis was performed for the leaf extract. Results:The chloroform fraction (ChF) was found to contain highest quantity of polyphenols (۱۱۴.۲۸ µg/ml of GAE), flavonoids (۹۵.۶۸ µg/ml of quercetin) and tannins (۶۳.۸۰ µg/ml of GAE) and also possessed effective inhibitory activity against α amylase (IC۵۰۹۵.۷۸ µg/ml) and α glucosidase (IC۵۰ ۱۲۶.۸۱ µg/ml). The antioxidant activity of ChFwas also higher when compared to other fractions. Further, GC-MS analysis of ChF showed the presence of various components that may be responsible for the above mentioned activities. Conclusion:The study findings suggest that the components present in the leaves of C. bonplandianus, may provide a potential therapeutic source in developing treatment forhyperglycemia. Further bioassay guided fractionation procedure is required to identify the active constituents.

کلیدواژه ها:

Antihyperglycemia ، α-Amylase ، α-Glucosidase ، Polyphenols ، Postprandial hyperglycemia

نویسندگان

Uma Dharshini Karuppiah Vijayamuthuramalingam