Establishment of human hair follicle mesenchymal stem cells with overexpressed human hepatocyte growth factor

سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 161

فایل این مقاله در 14 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_IJBMS-20-6_008

تاریخ نمایه سازی: 28 مهر 1400

چکیده مقاله:

Objective(s): Chronic liver disease has become a major health problem that causes serious damage to human health. Since the existing treatment effect was not ideal, we need to seek new treatment methods. Materials and Methods: We utilized the gene recombination technology to obtain the human hair mesenchymal stem cells which overexpression of human hepatocyte growth factor (hHGF). Furthermore, we verified the property of transfected cells through detecting surface marker by flow cytometry. Results: We show here establishment of the hHGF-overexpressing lentivirus vector, and successfully transfection to human hair follicle mesenchymal stem cells. The verified experiments could demonstrate the human hair follicle mesenchymal stem cells which have been transfected still have the properties of stem cells. Conclusion: We successfully constructed human hair follicle mesenchymal stem cells which overexpression hHGF, and maintain the same properties compared with pro-transfected cells.

کلیدواژه ها:

نویسندگان

Dan Zhou

Department of Pediatrics, The Second Hospital of Jilin University, Changchun ۱۳۰۰۴۱, China

Hongjing Cheng

Department of Gastroenterology, The First Hospital of Jilin University, Changchun ۱۳۰۰۴۱, China

Jinyu Liu

The Ministry of Education Key Laboratory, The College of Basic Medical Sciences, of Jilin University, Changchun ۱۳۰۰۴۱, China

Lei Zhang

Department of Pediatrics, The Second Hospital of Jilin University, Changchun ۱۳۰۰۴۱, China

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • Neuberger J. An update on liver transplantation: A critical review. J ...
  • Yan L, Cai C, Li J, Xu S, Chang Q, Li Y, Wu B.Present status and perspectives of ...
  • Ferrer JR, Chokechanachaisakul A, Wertheim JA. New tools in experimental cellular therapy for the treatment of ...
  • Zhou H, Liu H, Ezzelarab M, Schmelzer E, Wang Y, Gerlach J, Gridelli B, Cooper DK. Experimental ...
  • Bhatia SN, Underhill GH, Zaret KS, Fox IJ. Cell and tissue engineering for liver disease. ...
  • Ebrahimkhani MR, Neiman JA, Raredon MS, Hughes DJ, Griffith LG. Bioreactor technologies to support ...
  • Nakamura T, Mizuno S. The discovery of hepatocyte growth factor (HGF) ...
  • Gómez-Aristizábal A, Keating A, Davies JE.Mesenchymal stromal cells as supportive cells for hepatocytes. ...
  • Forte TM. Primary hepatocytes in mono-layer culture: a model for ...
  • Nakamura T۱, Sakai K, Nakamura T, Matsumoto K. Hepatocyte growth factor twenty years on: Much more ...
  • Kaido T, Oe H, Yoshikawa A, Okajima A, Imamura M. Expressions of ...
  • Atta H, El-Rehany M, Hammam O, Abdel-Ghany H, Ramzy M, ...
  • Kim MD, Kim SS, Cha HY, Jang SH, Chang DY, et al. ...
  • Seo KW, Sohn SY, Bhang DH, Nam MJ, Lee HW, Youn HY. ...
  • Xia JL, Dai C, Michalopoulos GK, Liu Y. Hepatocyte growth factor attenuates liver ...
  • Waters James M, Richardson Gavin D, Jahoda Colin AB. Hair ...
  • Anas El-Aneed. An overview of current delivery systems in cancer ...
  • Nakamura T, Nawa K and Ichihara A. Partial purification and ...
  • Gohda E, Tsubouchi H., Nakayama H, Hirono S, Sakiyama O,Takahashi ...
  • Zarnegar R, Michalopoulos G. Purification and biological characterization of human ...
  • Nakamura T, Nishizawa T, Hagiya M, Seki T, Shimonishi M, ...
  • Tashiro K, Hagiya M, Nishizawa T, Seki T, Shimonishi M, ...
  • Nakamura T. Structure and function of hepatocyte growth factor . ...
  • Matsumoto K, Nakamura T. Hepatocyte growth factor: molecular structure, roles ...
  • Birchmeier C, Gherardi E. Develop-mental roles of HGF/SF and its ...
  • Kellermann G, Boudechiche L, Weber A, Hadchouel M. Increased engraftment ...
  • Xue F, Takahara T, Yata Y, Kuwabara Y, Shinno E, ...
  • Yanagita K, Nagaike M, Ishibashi H, Niho Y, Matsumoto K, ...
  • Hu JJ, Sun C, Lan L, Chen YW, Li DG. ...
  • نمایش کامل مراجع