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مقالات فارسیISIکنفرانسهاژورنالها

Detection of Th۱۷/Treg cells and related factors in gingival tissues and peripheral blood of rats with experimental periodontitis

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 20، شماره: 3
سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 144

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استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1295760

شناسه ملی سند علمی:

JR_IJBMS-20-3_010

تاریخ نمایه سازی: 28 مهر 1400

چکیده مقاله:

Objective(s): This study aimed to investigate the role and the possible mechanisms involved in the immunoregulation of experimental periodontitis by Th۱۷/Treg. Materials and Methods: Experimental periodontitis was established by silk thread ligation with Porphyromonasgingivalis daubing in the bilateral maxillary second molar of Male Sprague-Dawley (SD) rats. Alveolar bones were scanned by Micro-CT. Histological examination was stained with H&E. The proportions of Th۱۷ and Treg cells in peripheral blood were detected by flow cytometry. RT-PCR was used to measure the expression of RORγt, Foxp۳ mRNA in the gingival tissues. The concentrations of IL-۱۷, IL-۱۰, and TGF-β in peripheral blood and gingival crevicular fluid were measured by ELISA. Results: Experimental rats showed profound bone resorption and inflammatory cell infiltration. The percentages of Th۱۷ significantly increased in the peripheral blood, which was consistent with gingival tissues study that Th۱۷ cells related transcription factor RORγt mRNA and IL-۱۷ increased in the course of periodontitis. The percentages of CD۲۵+Foxp۳+ Treg significantly increased in the peripheral blood, which was consistent with gingival tissues study that Treg cells related transcription factor Foxp۳ mRNA and cytokines IL-۱۰ and TGF-β increased in the course of periodontitis. The ratio of Th۱۷/Treg cells was significantly increased in the peripheral circulation, however, the Th۱۷/Treg balance is in wave motion in inflamed gingival tissues in the different stages of periodontitis. Conclusion: Th۱۷/Treg balance may be associated with the progression of periodontitis and pathological tissue destruction. Moreover, local inflammation would result in the up-regulation ratio of Th۱۷/Treg in peripheral blood, which may influence some periodontally involved systemic diseases.

کلیدواژه ها:

Bone resorption ، Cytokine ، Experimental periodontitis ، Th۱۷ ، Treg

نویسندگان

Li Gao

Department of Periodontology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat -sen University, Guangzhou ۵۱۰۰۵۵,China

Yajing Zhao

Guangdong Provincial Key Laboratory of Stomatology, Guangzhou ۵۱۰۰۵۵, China

Panpan Wang

Department of Periodontology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat -sen University, Guangzhou ۵۱۰۰۵۵,China

Liping Zhang

Guangdong Provincial Key Laboratory of Stomatology, Guangzhou ۵۱۰۰۵۵, China

Chi Zhang

Guangdong Provincial Key Laboratory of Stomatology, Guangzhou ۵۱۰۰۵۵, China

Qianying Chen

Guangdong Provincial Key Laboratory of Stomatology, Guangzhou ۵۱۰۰۵۵, China

Chuanjiang Zhao

Department of Periodontology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat -sen University, Guangzhou ۵۱۰۰۵۵,China

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