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مقالات فارسیISIکنفرانسهاژورنالها

CFP۱۰: mFcγ۲ as a novel tuberculosis vaccine candidate increases immune response in mouse

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 20، شماره: 2
سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 247

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لینک ثابت به این مقاله:
https://civilica.com/doc/1295768

شناسه ملی سند علمی:

JR_IJBMS-20-2_003

تاریخ نمایه سازی: 28 مهر 1400

چکیده مقاله:

Objective(s): Despite treatment with antibiotics and vaccination with BCG, tuberculosis (TB) is still considered as one of the most important public health problems in the world. Therefore, designing and producing a more effective vaccine against TB seems urgently. In this study, immunogenicity of a fusion protein which consisting or comprising CFP-۱۰ from Mycobacterium tuberculosis and the Fc-domain of  mouse IgG۲a was evaluated as a novel subunit vaccine candidate against TB. Materials and Methods: The genetic constructs were cloned in pPICZαA expression vector and recombinant vectors (pPICZαA-CFP-۱۰: Fcγ۲a and pPICZαA-CFP-۱۰:His) were transformed into Pichia pastoris. To evaluate the expression of recombinant proteins, SDS-PAGE and immunoblotting were used. The immunogenicity of recombinant proteins, with and without BCG were assessed in BALB/c mice and specific cytokines against recombinant proteins (IFN-γ, IL-۱۲, IL-۴, IL-۱۷ and TGF-β) were evaluated. Results: The levels of IFN-γ and IL-۱۲ in mice that received recombinant proteins was higher than the control groups (BCG and PBS). Thus, both recombinant proteins (CFP-۱۰:Fcγ۲a and CFP-۱۰:His) could excite good response in Th۱-cells. The Fc-tagged protein had a stronger Th۱ response with low levels of IL-۴, as compared to CFP-۱۰:His. However, the highest level of Th۱ response was observed in groups that were vaccinated with BCG (prime) and then received recombinant protein CFP-۱۰: Fcγ۲a (booster). Conclusion: The results demonstrated that binding mice Fc-domain to CFP-۱۰ protein can increase the immunogenicity of the subunit vaccine. Further studies, might be able to design and produce a new generation of subunit vaccines based on the Fc-fused immunogen.

کلیدواژه ها:

CFP-۱۰ ، Fcγ۲a ، Immunogenicity ، Mycobacterium tuberculosis ، Subunit vaccine

نویسندگان

Ali Asghar Baghani

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Saman Soleimanpour

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Hadi Farsiani

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Arman Mosavat

HIV/AIDS, HTLV and Viral Hepatitis Research Center, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad, Iran

Masoud Yousefi

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Zahra Meshkat

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Seyed Abdolrahim Rezaee

Immunology Research Center, Inflammation and Inflammatory Diseases Division, Medical school, Mashhad University of Medical Sciences, Mashhad, Iran

Saeid Amel Jamehdar

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Mohammad Reza Akbari Eydgahi

Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran

Hamid Sadeghian

Organic Chemistry, Department of Laboratory Sciences, Mashhad University of Medical Sciences, Mashhad, Iran

Kiarash Ghazvini

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

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