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مقالات فارسیISIکنفرانسهاژورنالها

The cytotoxic effect of memantine and its effect on cytoskeletal proteins expression in metastatic breast cancer cell line

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 20، شماره: 1
سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 208

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لینک ثابت به این مقاله:
https://civilica.com/doc/1295788

شناسه ملی سند علمی:

JR_IJBMS-20-1_007

تاریخ نمایه سازی: 28 مهر 1400

چکیده مقاله:

Objective(s):Breast cancer is an important leading cause of death from cancer. Stathmin and tau proteins are regulators of cell motility, and their overexpression is associated with the progression and bad prognosis of breast cancer. Memantine, an N-methyl-D-aspartate (NMDA) receptor antagonist, is the potential inhibitor of tau protein in neurons. This study determines the effect of memantine on breast cancer cell migration and proliferation, tau and stathmin gene expression in cancer cells and its synergistic effect with paclitaxel.   Materials and Methods: The cell proliferation was evaluated by MTT assay and for this purpose, MCF-۷ breast cancer cells were treated with various concentration of memantine (۲, ۲۰ and ۱۰۰ μg/ml). Tau and stathmin mRNA expression was evaluated through quantitative real time RT-PCR method. The migration of cancer cells treated with memantine for ۲۴ hr was compared to non-treated cells using an in vitro transmembrane migration assay. Results: Incubation of breast cancer cells with memantine resulted in a dose dependent reduction in cell survival (P=۰.۰۰۰۱). Paclitaxel (۱۰۰ nM) showed synergistic effect with memantine (P=۰.۰۰۰۱). Memantine significantly decreased tau and stathmin mRNA expression (by RT-PCR), so that ۱۰۰ µmol/l of memantine decreased tau and stathmin expression by ۴۶% (P=۰.۰۳۴۱) and ۳۳% (P=۰.۰۴۳), respectively. Migration of cells was also decreased by memantine (P=۰.۰۰۰۱). Conclusion: The presented data shows that memantine reduced mRNA levels of tau and stathmin proteins and also reduced cellular migration.

کلیدواژه ها:

Breast Cancer ، Memantine ، Metastasis ، Paclitaxel ، Stathmin ، Tau protein

نویسندگان

Sima Seifabadi

Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran

Golnaz Vaseghi

Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran

Shaghayegh Haghjooy Javanmard

Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran

Elham Omidi

Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran

Mohammadhasan Tajadini

Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran

Bahareh Zarrin

Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran

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  • National Cancer Institute SEER Stat Fact Sheets: Female breast cancer ...
  • Hermann PC, Huber SL, Herrler T, Aicher A, Ellwart JW, ...
  • Goodsell DS. The molecular perspective: micro-tubules and the taxanes. Oncologist ...
  • Quintá HR, Galigniana NM, Erlejman AG, Lagadari M, Piwien-Pilipuk G, ...
  • Jordan MA, Wilson L. Microtubules as a target for anticancer ...
  • Yang X, Kandil D, Cosar EF, Khan A. Fibroepithelial tumors ...
  • Mistry S, Atweh GF. Role of stathmin in the regulation ...
  • Marklund U, Larsson N, Gradin HM, Brattsand G, Gullberg M. ...
  • Mistry S, Luo XN, Atweh GF. Transcriptional regulation of phosphoprotein ...
  • Luo X, Mookerjee B, Ferrari A, Mistry S, Atweh GF. ...
  • Brattsand G. Correlation of oncoprotein ۱۸/stathmin expression in human breast ...
  • Goedert M, Spillantini MG, Jakes R, Rutherford D, Crowther RA. ...
  • Li ZH, Xiong QY, Tu JH, Gong Y, Qiu W, Zhang HQ, et al. Tau ...
  • Zhou J, Qian S, Li H, He W, Tan X, ...
  • Sontag E, Nunbhakdi-Craig V, Lee G, Bloom GS, Mumby MC. ...
  • Noble W, Planel E, Zehr C, Olm V, Meyerson J, ...
  • Wu TY, Chen CP. Dual action of memantine in Alzheimer ...
  • Degerman Gunnarsson M, Kilander L, Basun H, Lannfelt L. Reduction ...
  • Mowla SJ, Emadi Bayegi M, Ziaee SA, Nikpoor P. Evaluating ...
  • Kim SH, Sehrawat A, Singh SV. Notch۲ activation by benzyl ...
  • Matrone MA, Whipple RA, Thompson K, Cho EH, Vitolo MI, ...
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  • Trovik J, Wik E, Stefansson IM, Marcickiewicz J, Tingulstad S, ...
  • Miceli C, Tejada A, Castaneda A, Mistry SJ. Cell cycle ...
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