Genetic Profiling of Pseudomonas aeruginosa Isolates from Iranian Patients with Cystic Fibrosis Using RAPD-PCR and PFGE
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 12، شماره: 3
سال انتشار: 1388
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 138
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شناسه ملی سند علمی:
JR_IJBMS-12-3_001
تاریخ نمایه سازی: 30 مهر 1400
چکیده مقاله:
Objective(s) Pseudomonas aeruginosa is the most important cause of chronic lung infections and death in patients with cystic fibrosis. Determining the distribution of specific strains within patient populations is important in order to examine the epidemiology of the disease and the possibility of cross infection among patients. Materials and Methods Forty six Iranian patients with cystic fibrosis were studied for colonization with P. aeruginosa. Colony phenotype was recorded and antibiotic susceptibility to '' antibiotics was determined using the disc diffusion method. Genetic fingerprinting was carried out by RAPD-PCR and by PFGE. Results Forty five P. aeruginosa isolates were recovered from ۳۱ patients including sequential cultures from ۹ subjects. The rate of colonization increased with age. All isolates were susceptible to tobramycin and ciprofloxacin, ۹۷.۸% were sensitive to amikacin and piperacillin, ۹۳.۳% to gentamycin, ۹۱.۱% to ticarcillin, ۸۶.۷% to colistin, ۸۰% to carbenicillin, ۴۸.۹% to cefotaxime, ۲۶.۷% to imipenem and ۱۱.۱% to ceftazidime. Genetic fingerprinting showed similar distribution profiles for RAPD-PCR and PFGE and the majority of the isolates had unique fingerprints. Conclusion No relationship was observed between the obtained genotypes and antibiotic susceptibility profiles and common predominant virulent clones were not found among the isolates.
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نویسندگان
Fereshteh Eftekhar
Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, GC, Tehran, Iran
Nazanin Hosseinkhan
Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, GC, Tehran, Iran
Ahmad Asgharzadeh
Soil and Water Research Institute, Tehran, Iran
Ahmad Tabatabaii
Medical School, Shahid Beheshti University, Tehran, Iran
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