Chronic Effects of Di(۲-ethylhexyl)phthalate on Stereological Parameters of Testis in Adult Wistar Rats
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 13، شماره: 4
سال انتشار: 1389
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 212
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شناسه ملی سند علمی:
JR_IJBMS-13-4_002
تاریخ نمایه سازی: 3 آبان 1400
چکیده مقاله:
Objective(s) In recent years concerns have been raised regarding the incidence of male reproductive disorders from exposure to endocrine disruptors. So, chronic effects of di(۲-ethylhexyl)phthalate were studied on histological and stereological structure of testis in adult Wistar rats. Materials and Methods Thirty two adult Wistar rats were randomly divided in four equal experiment groups; oil vehicle group and three treated groups which received ۱۰, ۱۰۰ and ۵۰۰ mg/kg/day di(۲-ethylhexyl)phthalate by gavage for ۹۰ days, respectively. At the end of exposure period the volume of testes was measured by Cavellieri method, testes weight was recorded and then fixed in Bouin’s solution. Following tissue processing, ۵ pm sections were stained with haematoxylin-eosin and evaluated with quantitative techniques. Seminiferous tubule diameter, germinal epithelium height, relative and total volumes of seminiferous tubules, tubular lumen and interstitial tissue were estimated. Results The results showed that mean weight and volume of testis were decreased significantly (۳۵.۲% and ۲۳.۹% respectively) in rats treated with ۵۰۰ mg/kg/day DEHP for ۹۰ days. Seminiferous tubules diameter reduced, ۴.۴% and ۱۳.۴% in ۱۰۰ and ۵۰۰ mg/kg/day DEHP-treated groups, respectively. Relative volumes of tubular lumen and interstitial tissue were increased significantly in ۱۰۰ (P< ۰.۰۵) and ۵۰۰ (P< ۰.۰۱) mg/kg/day doses groups. Also, testosterone serum levels were significantly higher (P< ۰.۰۵) in rats exposed to ۵۰۰ mg/kg/day DEHP. Conclusion Present study indicated dose-dependent reductions of testicular parameters in adult male rats chronically exposed to di(۲-ethylhexyl)phthalate.
کلیدواژه ها:
نویسندگان
Mehran Dorostghoal
Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahwaz, Ahwaz,
Adel Zardkaf
Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahwaz, Ahwaz,
Abdolrahaman Dezfoolian
Department of Anatomical Sciences, Faculty of Medicine, Jondishapour Medical Sciences University of Ahwaz, Ahwaz, Iran
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