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مقالات فارسیISIکنفرانسهاژورنالها

Combination of Stem Cell Mobilized by Granulocyte-Colony Stimulating Factor and Human Umbilical Cord Matrix Stem Cell: Therapy of Traumatic Brain Injury in Rats

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 14، شماره: 4
سال انتشار: 1390
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 160

فایل این مقاله در 13 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1296996

شناسه ملی سند علمی:

JR_IJBMS-14-4_004

تاریخ نمایه سازی: 3 آبان 1400

چکیده مقاله:

Objective(s) Clinical studies of treating traumatic brain injury (TBI) with autologous adult stem cells led us to examine the impression of a combination therapy. This was performed by intravenous injection of human umbilical cord matrix stem cell (hUCMSC-Wharton,s jelly stem cell) with bone marrow cell mobilized by granulocytecolony stimulating factor (G-CSF) in rats injured with cortical compact device. Materials and Methods Adult male Wistar rats (n= ۵۰) were injured with controlled cortical impact device and divided into five groups. All injections were performed ۱ day after injury into the tail veins of rats. Neurological functional evaluation of animals was performed before and after injury using modified neurological severity scores (mNSS). Animals were sacrificed ۴۲ days after TBI and brain sections were stained by Brdu immunohistochemistry. Results Statistically significant improvement in functional outcome was observed in treatment groups when compared with control (P< ۰.۰۱). mNSS showed no significant differences among the hUCMSC and G-CSF treated groups at any time point (end of trial). Rats with hUCMSC + G-CSF treatment had a significant improvement on mNSS at ۵ and ۶ week compared to other treatment group (P< ۰.۰۱). Conclusion Histological analysis in G-CSF+ hUCMSC treated traumatic rats exhibited significant increase in numbers of Brdu immunoreactive cells in their traumatic core compared with other labeled group.

کلیدواژه ها:

Combine Therapy ، G-CSF ، Stem cell ، TBI ، Whartons jelly

نویسندگان

Mehrdad Bakhtiary

Cellular & Molecular Research Center, Tehran University of Medical Sciences, Tehran, Iran

Mohsen Marzban

Cellular & Molecular Research Center, Tehran University of Medical Sciences, Tehran, Iran

Mehdi Mehdizadeh

Cellular & Molecular Research Center, Tehran University of Medical Sciences, Tehran, Iran

Mohammad Taghi Joghataei

Cellular & Molecular Research Center, Tehran University of Medical Sciences, Tehran, Iran

Samideh Khoei

Cellular & Molecular Research Center, Tehran University of Medical Sciences, Tehran, Iran

Mahdi Tondar

Department of Anatomy, Tehran University of Medical Science, Tehran, Iran

Vahid Pirhajati Mahabadi

Department of Anatomy, Tehran University of Medical Science, Tehran, Iran

Bahareh Laribi

Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran

Asghar Ebrahimi

Department of Biotechnology and Bioscience, Milano-Bicocca University, Milan, Italy

Seyed Jafar Hashemian

Department of Anatomy, Tehran University of Medical Science, Tehran, Iran

Navid Modiry

Department of Anatomy, Tehran University of Medical Science, Tehran, Iran

Soraya Mehrabi

Department of physiology, Tehran University of Medical Sciences, Tehran, Iran

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