Production of Recombinant Adenovirus Containing Human Interlukin-۴ Gene
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 14، شماره: 6
سال انتشار: 1390
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 122
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شناسه ملی سند علمی:
JR_IJBMS-14-6_009
تاریخ نمایه سازی: 3 آبان 1400
چکیده مقاله:
Objective(s) Recombinant adenoviruses are currently used for a variety of purposes, including in vitro gene transfer, in vivo vaccination, and gene therapy. Ability to infect many cell types, high efficiency in gene transfer, entering both dividing and non dividing cells, and growing to high titers make this virus a good choice for using in various experiments. In the present experiment, a recombinant adenovirus containing human IL-۴ coding sequence was made. IL-۴ has several characteristics that made it a good choice for using in cancer gene therapy, controlling inflammatory diseases, and studies on autoimmune diseases. Materials and Methods In brief, IL-۴ coding sequence was amplified by PCR and cloned in pAd-Track-CMV. Then, by means of homologous recombination between recombinant pAd-Track-CMV and Adeasy-' plasmid in bacteria, recombinant adenovirus complete genome was made and IL-۴ containing shuttle vector was incorporated into the viral backbone. After linearization, for virus packaging, viral genome was transfected into HEK-۲۹۳ cell line. Viral production was conveniently followed with the aid of green fluorescent protein. Results Recombinant adenovirus produced here, was capable to infecting cell lines and express interlukin-۴ in cell. Conclusion This system can be used as a powerful, easy, and cost benefit tool in various studies on cancer gene therapy and also studies on immunogenetics.
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نویسندگان
Majid Mojarrad
Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Yassan Abdolazimi
Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Jamshid Hajati
Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Mohammad Hossein Modarressi
Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
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