Effect of selegiline on neural stem cells differentiation: a possible role for neurotrophic factors
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 18، شماره: 6
سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 135
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شناسه ملی سند علمی:
JR_IJBMS-18-6_004
تاریخ نمایه سازی: 3 آبان 1400
چکیده مقاله:
Objective(s): The stimulation of neural stem cells (NSCs) differentiation into neurons has attracted great attention in management of neurodegenerative disease and traumatic brain injury. It has been reported that selegiline could enhance the morphologic differentiation of embryonic stem cells. Therefore this study aimed to investigate the effects of selegiline on NSCs differentiation with focus on the role of neurotrophic factor gene expression. Materials and Methods: The NSCs were isolated from lateral ventricle of C۵۷ mice brain. The cells were exposed to selegiline in nano to micromolar concentrations for ۲۴ hr or ۷۲ hr. In order to assay the effect of selegiline on NSCs differentiation into neurons, astrocytes and oligodendrocytes, immunocytochemical techniques were utilized. Samples were exposed to specific antibodies against neurons (β tubulin), astrocytes (GFAP) and oligodendrocytes (OSP). The expression of BDNF, NGF and NT۳ genes was investigated using Real-Time PCR. Results: Our findings revealed that selegiline increased NSCs differentiation into neurons at ۱۰-۷ and ۱۰-۸ M and decreased the differentiation into astrocytes at ۱۰-۹,while oligodendrocyte did not significantly change in any of the used concentrations. In addition data analyses showed that selegiline increased BDNF, NGF and NT۳ gene expression at ۲۴ hr, but did not change them in the other time of exposure (۷۲ hr) except ۱۰-۷ M concentration of selegiline, which increased NT۳ expression. Conclusion:Our results indicate selegiline induced the differentiation of NSCs into neurons and in this context the role of neurotrophic factors is important and should be considered.
کلیدواژه ها:
نویسندگان
Kambiz Hassanzadeh
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
Mehrnoush Nikzaban
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
Mohammad Raman Moloudi
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
Esmael Izadpanah
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
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