Role of Oxidative Stress in Ethanol-induced Neurotoxicity in the Developing Cerebellum
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 15، شماره: 4
سال انتشار: 1391
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 205
فایل این مقاله در 10 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJBMS-15-4_010
تاریخ نمایه سازی: 4 آبان 1400
چکیده مقاله:
Objective(s) The purpose of this study was to investigate the role of oxidative stress in Purkinje cell neurotoxicity ofethanol-treated rat. Materials and Methods Male rat pups ۴-day-old was used in this study. Ethanol was administered to rat pups at a dose of ۶ g/kg from postnatal days (PDs) ۴ to ۵. Pups were killed ۹۰ min after the second alcohol treatment on PD ۵ by decapitation and the brain was immediately removed. The cerebellum was dissected for analyzing the oxidative stress parameters and histological study. The activities of several antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in vermis of cerebellum were assayed. Thiobarbituric acid reactive substances (TBARS) levels were also measured as a marker of lipid peroxidation. Results Administration of ethanol significantly increased TBARS levels in the cerebellum compared to control pups (P< ۰.۰۱). The treated pups with ethanol exhibited a marked decrease in the GPx activity (P< ۰.۰۱) whereas, in spite of decrease in the activities of SOD and CAT, when compared to control, there were not significant differences. The spherical cell bodies of Purkinje cells in control rats are aligned nicely between the granular and molecular layers. In ethanol treated pups, Purkinje cells scattered within the Purkinje cell layer and shrinkage of the cell somata is seen. Conclusion The results of the present work demonstrated that ethanol exposure during the vulnerable window could increase TBARS levels (lipid peroxidation) and decrease GPx levels in pup's cerebellum. Also, the results confirmed ethanol-induced microencephaly, cerebellar Purkinje cell loss. These findings suggest that Purkinje cell loss is, in part through decrease in the activity of GPx and increase of lipid peroxidation in the rat cerebellum.
کلیدواژه ها:
نویسندگان
Azam Ramezani
Faculty of Biology, Damghan University, Damghan, Iran
Iran Goudarzi
Faculty of Biology, Damghan University, Damghan, Iran
Taghi Lashkarboluki
Faculty of Biology, Damghan University, Damghan, Iran
Mohammad Taghi Ghorbanian
Faculty of Biology, Damghan University, Damghan, Iran
Kataneh Abrari
Faculty of Biology, Damghan University, Damghan, Iran
Mahmoud Elahdadi Salmani
Faculty of Biology, Damghan University, Damghan, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :