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The role of local renin-angiotensin system on high glucose-induced cell toxicity, apoptosis and reactive oxygen species production in PC۱۲ cells

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 17، شماره: 8
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 212

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استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1297829

شناسه ملی سند علمی:

JR_IJBMS-17-8_012

تاریخ نمایه سازی: 4 آبان 1400

چکیده مقاله:

Objective(s): Hyperglycemia, oxidative stress and apoptosis have key roles in pathogenesis of diabetic neuropathy. There are local renin-angiotensin systems (RASs) in different tissues such as neural tissue. Local RASs are involved in physiological and pathophysiological processes such as inflammation, proliferation and apoptosis. This study aimed to investigate the role of local renin-angiotensin system on high glucose-induced cell toxicity, apoptosis and reactive oxygen species (ROS) production in PC۱۲ cells, as a cell model of diabetic neuropathy. Materials and Methods: PC۱۲ cells were exposed to a high glucose concentration (۲۷ mg/ml), captopril (ACE inhibitor), telmisartan and losartan (AT۱ antagonists), and also PD۱۲۳۳۱۹ (AT۲ antagonist) were administered before and after induction of high glucose toxicity. Then cell viability was assessed by MTT assay and apoptotic cells and intracellular ROS production were detected by annexin V-propidium iodide and DCFDA, respectively, using flow cytometry. Results: High glucose concentration decreased cell viability, and increased apoptotic cells. Intracellular ROS production was also increased. In PC۱۲ cells pretreatment and treatment by the drugs showed a significant improvement in cell viability and reduced apoptosis in captopril, telmisartan and PD۱۲۳۳۱۹ but only captopril and telmisartan were able to reduce ROS production. Losrtan significantly lowered ROS but didn’t show any improvements in cell viability and apoptotic cells. Conclusion:  The results of the present study showed that RAS inhibitors reduced cell toxicity and apoptosis and ROS production was induced by high glucose. It may be suggested that local RAS has a role in high glucose toxicity.

کلیدواژه ها:

Apoptosis ، High glucose toxicity ، Oxidative stress ، PC۱۲ ، Renin-angiotensin system

نویسندگان

Kaveh Shahveisi

Neurocognitive Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ۲ Sleep Disorders Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Seyed Hadi Mousavi

Pharmacological Research Center of Medicinal Plants and Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Mahmoud Hosseini

Neurocognitive Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Abolfazl Khajavi Rad

Applied Physiology Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Seyed Amir Jalali

Immunogenetic and Cell Culture Department, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran North Khorasan University of Medical Sciences, Bojnurd, Iran

Ziba Rajaei

Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Hamid Reza Sadeghnia

Neurocognitive Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Mousa-Al-Reza Hadjzadeh

Applied Physiology Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

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  • Hsueh WA, Wyne K. Renin-Angiotensin-aldosterone system in diabetes and hypertension. ...
  • Steckelings UM, Rompe F, Kaschina E, Unger T. The evolving ...
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  • Coppey LJ, Davidson EP, Rinehart TW, Gellett JS, Oltman CL, ...
  • Piga R, Naito Y, Kokura S, Handa O, Yoshikawa T. ...
  • Sachse A, Wolf G. Angiotensin II-induced reactive oxygen species and ...
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  • Yu T, Robotham JL, Yoon Y. Increased production of reactive ...
  • Pop-Busui R, Sima A, Stevens M. Diabetic neuropathy and oxidative ...
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  • Paul M, Poyan Mehr A, Kreutz R. Physiology of local ...
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  • Herr D, Bekes I, Wulff C. Local Renin-Angiotensin System in ...
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  • Wolf G. "The road not taken": role of angiotensin II ...
  • Yorek MA. The potential role of angiotensin converting enzyme and ...
  • Forstermann U. Oxidative stress in vascular disease: causes, defense mechanisms ...
  • Yamagishi S, Amano S, Inagaki Y, Okamoto T, Inoue H, ...
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  • Wolf G, Wenzel U, Burns KD, Harris RC, Stahl RA, ...
  • Kamper M, Tsimpoukidi O, Chatzigeorgiou A, Lymberi M, Kamper EF. ...
  • Bravenboer B, Kappelle AC, Hamers FP, van Buren T, Erkelens ...
  • Allen DA, Yaqoob MM, Harwood SM. Mechanisms of high glucose-induced ...
  • Brownlee M. Biochemistry and molecular cell biology of diabetic complications. ...
  • Brownlee M. The pathobiology of diabetic complications: a unifying mechanism. ...
  • Kasznicki J, Kosmalski M, Sliwinska A, Mrowicka M, Stanczyk M, ...
  • Malik RA, Williamson S, Abbott C, Carrington AL, Iqbal J, ...
  • Singh VP, Le B, Khode R, Baker KM, Kumar R. ...
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