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The Effect of Platelet Rich Plasma on Chondrogenic Differentiation of Human Adipose Derived Stem Cells in Transwell Culture

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 16، شماره: 11
سال انتشار: 1392
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 157

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لینک ثابت به این مقاله:
https://civilica.com/doc/1298110

شناسه ملی سند علمی:

JR_IJBMS-16-11_007

تاریخ نمایه سازی: 4 آبان 1400

چکیده مقاله:

  Objective(s): Platelet-rich plasma (PRP) has recently emerged as a promising strategy in regenerative medicine due to its multiple endogenous growth factors. Little is known about the role of PRP as a promoter in chondrogenesis of human adipose derived stem cells (hADSCs). The aim of this study was to determine whether PRP may be considered as a natural and easy achievable source of growth factors to promote the chondrogenic differentiation of hADSCs in Transwell culture.   Materials and Methods : Biochemical, immunohistological and molecular assays were used to evaluate the effect of different concentrations (۵%, ۱۰%, and ۱۵%) of PRP on chondrogenic differentiation of hADSCs in Transwell culture. Results : The cells in the presence of ۱۰% PRP produced markedly higher amounts of GAG and DNA, in comparison to the control group. PRP also increased chondrogenic markers in these cells, such as sox-۹, aggrecan and collagen type II. A high expression level of collagen type X as a hypertrophic marker was observed in cartilage produced by using either PRP or TGF-β۱. Conclusion : Our findings indicate that autologous PRP at an optimum concentration had beneficial effects on differentiation of hADSCs in Transwell culture. Further, in vivo studies are necessary to fully define the clinical implications of PRP.

کلیدواژه ها:

Adipose-derived stem cells Platelet-rich plasma Transwell culture

نویسندگان

Mohammad Mardani

Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Azadeh Kabiri

Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran ۲ Department of Anatomical Sciences, Paramedical school, Guilan University of Medical Sciences, Langeroud, Iran

Ebrahim Esfandiari

Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abolghasem Esmaeili

Cell, Molecular and Developmental Biology Division, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran

Abbasali Pourazar

Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan Iran

Malekmassoud Ansar

Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.Department of Anatomical Sciences, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran

Batool Hashemibeni

Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

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