The Effect of Androgen Deprivation on the Expression of Connexin-۴۳ mRNA in the Heart
محل انتشار: مجله سلول و تحقیقات مولکولی، دوره: 13، شماره: 1
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 160
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شناسه ملی سند علمی:
JR_JCMR-13-1_008
تاریخ نمایه سازی: 22 آذر 1400
چکیده مقاله:
Connexin-۴۳ (Cx-۴۳) plays axial roles in the propagation of action potentials and contractile coupling in heart. Down-regulation of Cx-۴۳ in heart is associated with arrhythmia, dilated cardiomyopathy and heart failure. To date, no studies have examined the effects of androgen deprivation therapy (ADT)-induced hypogonadism on the expression of Cx-۴۳ in heart. This study investigated the effects of testosterone deprivation and its replacement with testosterone on the expression of Cx-۴۳ mRNA and muscle-specific miRNAs miR-۲۰۶ and miR-۱, as two potential regulators of the Cx-۴۳ protein expression in the ventricular tissue. Accordingly, ۲۱ male Wistar rats were divided into three groups: Ι) Normal control, П) ORX-S: castrated rats serving as animal models for ADT and receiving the sesame oil as a solvent of testosterone enanthate for ۱۰ weeks, and Ш) ORX-T: these animals were castrated, receiving testosterone enanthate (۲۵ mg/kg) for ۱۰ weeks. The relative expression of Cx-۴۳ mRNA, miR-۲۰۶ and miR-۱ was determined by qRT-PCR. Cx-۴۳ mRNA was found to be decreased in the ORX-S group. The Cx-۴۳ mRNA was up-regulated after the administration of testosterone enanthate. There were no significant changes in miR-۲۰۶ and miR-۱ levels in the ORX-S and ORX-T groups when compared to the controls. Our results indicated that testosterone should be regarded as an important factor in the regulation of the Cx-۴۳ mRNA expression in heart, and testosterone deprivation may down-regulate the Cx-۴۳ mRNA expression; however, it doesn’t alter miR-۱ and miR-۲۰۶ levels. These results suggest that ADT-induced hypogonadism may put males at risk for cardiac dysfunctions.
کلیدواژه ها:
نویسندگان
Mahnaz Ghowsi
Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran
Nazli Khajehnasiri
Department of Biological Sciences, Faculty of Basic Sciences, Higher Education Institute of Rab-Rashid, Tabriz, Iran.
Sajjad Sisakhtnezhad
Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran
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