Association of novel polymorphisms in follicle stimulating hormone beta (FSHβ) gene with litter size in Mehraban sheep

سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 277

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شناسه ملی سند علمی:

JR_KLST-9-2_003

تاریخ نمایه سازی: 2 بهمن 1400

چکیده مقاله:

Follicle-stimulating hormone (FSH) is necessary for the hypothalamic-pituitary-gonadal axis, and plays an important role in reproduction by binding to a specific receptor (FSHR) through β-subunit of FSH in the surface of the ovarian granulosa cells. This study aimed to characterize FSH β-subunit gene (FSHβ) polymorphism and its association with litter size (LS) using a sample of ۱۱۸ Mehraban sheep. Polymerase chain reaction (PCR) was performed to amplify two fragments of ۳۰۰ bp and ۴۳۱ bp of the ovine FSHβ gene (Oar_v۴.۰; Chr ۱۵, NC_۰۱۹۴۷۲.۲). Polymorphisms in the studied fragments were then explored using single strand conformational polymorphism (SSCP) and DNA sequencing methods. A total of seven single-nucleotide polymorphisms (SNPs), including g.۵۹۰۷۸۵۶۴ C>G, g.۵۹۰۷۸۶۲۴ T>C, g.۵۹۰۷۸۶۵۵ T>C, g.۵۹۰۷۸۶۹۱ T>C, g.۵۹۰۷۸۷۵۴ C>A, g.۵۹۰۸۰۱۸۶ G>C and g.۵۹۰۸۰۳۶۵ C>T, were found among the six detected SSCP patterns A to F. Moreover, two novel indel polymorphisms called e.g., g.۵۹۰۷۸۷۰۲del۸-bp−ins۶۴-bp and g.۵۹۰۷۸۷۲۶ins۵۴-bp were identified among the three different SSCP genotypes patterns G to I. We found significant differences on prolificacy categories between SSCP genotypes patterns D, E and F (P < ۰.۰۱) that simultaneously represented SNP polymorphisms of g.۵۹۰۷۸۷۵۴ C>A, g.۵۹۰۸۰۱۸۶ G>C and g.۵۹۰۸۰۳۶۵ C>T. Similarly, novel indel polymorphisms revealed a significant difference on prolificacy categories between SSCP genotypes patterns G, H and I (P < ۰.۰۵). Our results suggest that the FSHβ is a strong candidate gene to associate with the LS in sheep.

نویسندگان

Zahra Mokhtari

Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

Ahmad Ahmadi

Department of Animal Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan - IRAN

Pouya Zamani

Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

Reza Talebi

Department of Systems and Synthetic Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Mohammad Reza Ghafari

Department of Systems and Synthetic Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

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