Solution for Root Canal Treatment Failure: Comparison of Antibiofilm between Aloe Vera Extracts and Chitosan Shrimp Shells of the Formation Biofilm Enterococcus Faecalis

Enterococcus faecalis is a resistant bacterium in the root canal that can cause root canal treatment failure and is frequently found in root canal infections due to its ability to form biofilm. As a natural ingredient, aloe vera extract and chitosan can be used as an antibiofilm. This study aims to compare the antibiofilm properties of aloe vera extract and chitosan shrimp shells. The antibiofilm test was divided into two groups: groups I and II, tested ۲۴ h and ۷۲ h after biofilm formation by adding ۰.۱ mL of aloe vera suspension and ۰.۱ mL of chitosan suspension in ۹۶ well plates containing E. faecalis. The aloe vera extract and chitosan concentrations used were ۵۰%, ۲۵%, ۱۲.۵%, and ۶.۲۵%. ۰.۱% crystal violet staining and OD values ​​were measured using an ELISA reader. In the first group, aloe vera extracts at ۲۵% concentration were more significant on positive control and had a percentage of biofilm inhibition of ۹۳.۷۷%, whereas chitosan at ۲۵% concentration was most significant on positive control and had a percentage of biofilm inhibition of ۹۰.۵۹%. In the second group, aloe vera extracts of ۱۲.۵% concentration were most significant on positive control and had a percentage of biofilm inhibition of ۹۰.۶۸%, while chitosan at ۲۵% concentration was most significant on positive control and had a percentage of biofilm inhibition of ۸۸.۳۷%. Aloe vera extract at a concentration of ۱۲.۵% can inhibit the growth of Enterococcus faecalis biofilms more effectively compared with that of the shrimp shell chitosan.