Research Article: Chlorella growth factor extraction and its effect on gene expression of types
محل انتشار: مجله علوم شیلات ایران، دوره: 20، شماره: 6
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 110
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شناسه ملی سند علمی:
JR_JIFRO-20-6_008
تاریخ نمایه سازی: 23 بهمن 1400
چکیده مقاله:
Skin protects the body from outer factors as a barrier, and contains an important cosmetic role. Skin aging is related to collagen degradation and increasing multiple enzymes, including matrix metalloproteinase (MMPS), which degrade collagen. Chlorella vulgaris is a marine alga that exhibits anti-aging activity. The beneficial effects of Chlorella on skin make it a proper ingredient to be used in anti-aging products. In this study, the effect of Chlorella growth factor (CGF) on types І and ІІІ collagen in human fibroblast cell line Hu۰۲ was investigated. To find an effective extraction method in the present study, CGF was extracted using hot water extraction, enzymatic hydrolysis, and ultrasonication plus enzymatic hydrolysis, and the yields were compared. The yield of ultrasonication plus enzymatic hydrolysis method (CGF-۳) had the strongest absorbance at ۲۶۰ nm and highest solid recovery, compared to the other two methods. Using quantitative PCR, it was confirmed that CGF increased collagens І and ІІІ expression in skin fibroblast cells. This finding indicated that CGF induced collagen synthesis in Hu۰۲ cells. Gene expression of types І and ІІІ collagen were elevated to ۳.۱۴۴-fold and ۱.۱۴-fold in the CGF-۳ group, respectively, comparing to the control group.
کلیدواژه ها:
نویسندگان
S. Abdolbaghian
۱- Department of Marine Science, Faculty of Natural Resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Sh. Jamili
Iranian Fisheries Research Organization, Tehran, Iran
A. Manayi
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Science, Tehran, Iran
A. Mashinchian Moradi
۱- Department of Marine Science, Faculty of Natural Resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran.
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