Research Article: Molecular identification and toxicity effects of cyanobacteria species isolated from the Khoor-e-Khooran mangrove forest, Persian Gulf
محل انتشار: مجله علوم شیلات ایران، دوره: 20، شماره: 2
سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 129
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شناسه ملی سند علمی:
JR_JIFRO-20-2_020
تاریخ نمایه سازی: 23 بهمن 1400
چکیده مقاله:
The increasing incidence of the harmful cyanobacterial blooms in mangrove ecosystem is a potential threat for aquatic organisms and their consumers. In the present study, we have evaluated the biodiversity of cyanobacteria and monitored their cyanotoxins. We isolated ۱۲۰ bacterial isolates using BG۱۱ medium from water and sediment samples collected from ۱۰ stations throughout of the Khoor-e-Khooran mangrove forest at august ۲۰۱۸. Biodiversity and distribution pattern based on morphological characteristics showed that ۱۰ cyanobacterial genera were spread over the studied area. Phormidium, Oscillatoria, Spirulina and Nostoc genera were dominated with frequency percentages of ۲۵%, ۲۰%, ۱۰%, and ۱۰% respectively. Analysis of ۱۶S rRNA sequences showed that the strains have high similarity with type strains in NCBI GenBank ranged from ۹۸% to ۱۰۰%. Phylogenetic analysis proposed the non-indigenous origin of Microcystis strains because of their phylogenetic divergence. We detected microcystin gene in Microcystis sp. strain KH ۳, Microcystis sp. strain KH ۴ and Microcystis sp. strain KH ۱۱, while nodularin and cylindrospermopsin gene were not detected in all isolated cyanobacteria. The extracted metabolites from KH ۳ and KH ۴ strains showed cytotoxicity with LC۵۰ of ۱۳۹.۳ and ۲۲۵.۸ µg/mL against Artemia salina respectively. Their LC۵۰ were ۲۳۱.۳ and ۲۱۱.۲ µg/mL against shrimp larvae respectively. They inhibited the proliferation of HUVEC cell lines with IC۵۰ of ۱۱.۱۳ and ۱۳.۲۹ µg/mL and HEPG۲ with IC۵۰ of ۱۵.۴۹ and ۱۲.۵۱ µg/mL, respectively. Our results represented diversity and distribution pattern of cyanobacteria and demonstrated the incidence of microcystin in the Khoor-e-Khooran mangrove forest.
کلیدواژه ها:
نویسندگان
A. Zaheri
Department of Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
N. Bahador
Department of Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
M. Yousefzadi
Department of Marine Biology, Faculty of Marine Sciences and Technology, University of Hormozgan, Bandar Abbas, Iran
M. Arman
Department of Biology, Payame Noor University, PO BOX ۱۹۳۹۵-۳۶۹۷ Tehran, IRAN
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