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مقالات فارسیISIکنفرانسهاژورنالها

Rebaudioside A Enhances LDL Cholesterol Uptake in HepG۲ Cells via Suppression of HMGCR Expression

محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 10، شماره: 3
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 111

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استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1399085

شناسه ملی سند علمی:

JR_RBMB-10-3_017

تاریخ نمایه سازی: 25 بهمن 1400

چکیده مقاله:

Background: Rebaudioside A is one of the major diterpene glycosides found in Stevia had been reported to possess anti-hyperlipidemic effects. In this study, we explore the potential cholesterol-regulating mechanisms of Rebaudioside A in the human hepatoma (HepG۲) cell line in comparison with simvastatin. Methods: Cells were incubated with Rebaudioside A at several concentrations (۰-۱۰ μM) to determine the cytotoxicity by the MTT assay. Cells were treated with selected dosage (۱ and ۵ μM) in further experiments. Total cellular lipid was extracted by Bligh and Dyer method and subjected to quantitative colorimetric assay. To illustrate the effect of Rebaudioside A on cellular lipid droplets and low-density lipoprotein receptors, treated cells were subjected to immunofluorescence microscopy. Finally, we investigated the expression of experimental gene patterns of cells in response to treatment. Results: In this study, cytotoxicity of Rebaudioside A was determined at ۲۷.۷۲ μM. Treatment of cells with a higher concentration of Rebaudioside A promotes better hepatocellular cholesterol internalization and ameliorates cholesterol-regulating genes such as HMGCR, LDLR, and ACAT۲. Conclusions: In conclusion, our data demonstrated that Rebaudioside A is capable to regulate cholesterol levels in HepG۲ cells. Hence, we proposed that Rebaudioside A offers a potential alternative to statins for atherosclerosis therapy.

کلیدواژه ها:

Rebaudioside A ، Anti-hypercholesterolemia ، Lipid droplets ، Low-density lipoprotein ، HMGCR.

نویسندگان

Amirul Nazhan Ilias

Department of Veterinary Preclinical Science, Faculty of Veterinary Medicine, Universiti Putra Malaysia, ۴۳۴۰۰ UPM, Selangor, Malaysia.

Intan Safinar Ismail

Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, ۴۳۴۰۰ UPM, Selangor, Malaysia.

Hazilawati Hamzah

Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, ۴۳۴۰۰ UPM, elangor, Malaysia.

Taznim Begam Mohd Mohidin

Institute of Biological Sciences, Faculty of Science, University of Malaya, ۵۰۶۰۳ Kuala Lumpur, Malaysia.

Mohd Faiz Idris

Pusat Bahasa dan Pengajian Umum, Universiti Pendidikan Sultan Idris, ۳۵۹۰۰ Tanjong Malim, Perak, Malaysia.

Mokrish Ajat

Department of Veterinary Preclinical Science, Faculty of Veterinary Medicine, Universiti Putra Malaysia, ۴۳۴۰۰ UPM, Selangor, Malaysia.

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