More Related Gene Pathways to Vincristine-Induced Death Events in a Human T-Acute Lymphoblastic Leukemia Cell Line
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 114
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شناسه ملی سند علمی:
JR_RBMB-10-4_004
تاریخ نمایه سازی: 25 بهمن 1400
چکیده مقاله:
Background: Acute lymphoblastic leukemia (ALL) is common in children but rare in adults. Vincristine (VCR) is one of the drugs used at the beginning of treatment. Some genes are resistant to VCR in B-ALL.
Methods: Here, we examined the effect of VCR on gene expression changes in a T-ALL cell line, Jurkat. The MTT method was used to determine the IC۵۰ in Jurkat cells treated with different concentrations of VCR for ۴۸ and ۷۲ hours. Total RNA was isolated from the cells and cDNA was prepared. The Human
Cancer Drug Target PCR Array kit was used to evaluate the ۸۴ gene expression changes in Jurkat cells. Protein-protein interaction was analyzed by STRING software.
Results: We identified ۶۶ differentially expressed genes as comparison to untreated cells. The response to VCR-induced apoptotic events was remarkable in the pathways of heat shock protein, topoisomerase, protein kinases, cathepsins and cell cycle. In other pathways, there were resistant genes as well as sensitive genes to VCR treatment. Some proteins like HSP۹۰AA۱ and ESR۱ had determining associations with other proteins.
Conclusions: The results suggest VCR target genes in T-ALL cells may be beneficial biomarkers for ALL treatment and can be used to select appropriate synergistic drugs for VCR.
کلیدواژه ها:
نویسندگان
Azam Rashidbaghan
Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Ali Mostafaie
Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Yaghoub Yazdani
Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
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