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مقالات فارسیISIکنفرانسهاژورنالها

Evaluation of Radiotherapy on miR-۳۷۴ Gene Expression in Colorectal Cancer Patient Blood Samples

محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 10، شماره: 4
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 183

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این مقاله در بخشهای موضوعی زیر دسته بندی شده است:

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1399135

شناسه ملی سند علمی:

JR_RBMB-10-4_011

تاریخ نمایه سازی: 25 بهمن 1400

چکیده مقاله:

Background: Current cancer treatments include surgery, radiotherapy, chemotherapy, and immunotherapy. Despite these treatments, a main issue in cancer treatment is early detection. microRNAs (miRNAs) can be used as markers to diagnose and treat cancers. This study investigated the effect of radiotherapy on miR-۳۷۴ expression, and APC and GSK-۳β, two of its target genes, in the WNT pathway, in peripheral blood samples from radiotherapy-treated colorectal cancer (CRC) patients. Methods: Peripheral blood was collected from ۲۵ patients before and after radiotherapy. RNA was extracted from the blood and cDNA synthesized. miR-۳۷۴, APC, and GSK-۳β expression was determined by real-time polymerase chain reaction (RT-PCR) and the amplicons were sequenced. Finally, the data were statistically evaluated. Results: Quantitative RT-PCR revealed significant down-regulation of miR-۳۷۴ (۰.۶۳-fold) and upregulation of APC (۱.۱۲-fold) and GSK-۳β (۱.۲۲-fold) in CRC patients after five weeks of radiotherapy. Sequencing of PCR-produced amplicons confirmed the conservation of mature and precursor sequences encoding miR-۳۷۴. miR-۳۷۴ expression changed with time after radiotherapy treatment and related tumor grading. Increased age and tumor grade positively correlated with decreased miR-۳۷۴ expression. Conclusions: miR-۳۷۴ expression, and that of its two target genes, APC and GSK-۳β, changed after radiotherapy. These genes can likely be used as  diagnostic radiotherapy markers in CRC.

کلیدواژه ها:

Biomarker ، Colorectal cancer ، Mir-۳۷۴ ، Radiotherapy.

نویسندگان

Azam Ahmadi

Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran.

Mohamd Reza Bayatiani

Department of Medical Physics and Radiotherapy, Arak University of Medical Sciences and Khansari Hospital, Arak, Iran.

Fatemeh Seif

Department of Medical Physics and Radiotherapy, Arak University of Medical Sciences and Khansari Hospital, Arak, Iran.

Jamshid Ansari

Department of Medical Physics and Radiotherapy, Arak University of Medical Sciences and Khansari Hospital, Arak, Iran.

Parisa Rashidi

Department of Medical Physics and Radiotherapy, Arak University of Medical Sciences and Khansari Hospital, Arak, Iran.

Mona Moghadasi

Students Research Committee, Arak University of Medical Sciences, Arak, Iran.

Mobarakeh Etemadi

Students Research Committee, Arak University of Medical Sciences, Arak, Iran.

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