The pretreatment potential effects of omy-miR-۷۳۱ Antagomir in brain tissue of rainbow trout Oncorhynchus mykiss infected with viral hemorrhagic septicemia virus
محل انتشار: مجله علوم شیلات ایران، دوره: 19، شماره: 3
سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 94
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شناسه ملی سند علمی:
JR_JIFRO-19-3_040
تاریخ نمایه سازی: 27 بهمن 1400
چکیده مقاله:
Among the viral diseases in trout farming, viral hemorrhagic septicemia (VHS) could be mentioned as the most devastating worldwide viral infection. In the present investigation, the influence of the omy-miR-۷۳۱ Antagomir pretreatment on VHS in rainbow trout was examined. Trout (۲۰±۱) g were examined in four main groups subdivided into three subgroups, including a negative control without any injection, a vehicle that received saline, a positive control that was infected by VHSV, and an experimental group that received omy-miR-۷۳۱ Antagomir by injection, then they were challenged by VHSV. The survival rate, average weight gain, histopathology, Immunohistochemistry and Blood-brain barrier impermeability were assessed in each group. The survival rate and average weight gain in the positive control group was significantly decreased in comparison with other groups. Remarkable histopathological changes and virus detection by immunohistochemistry test were observed in the positive control, whereas no sign of the virus was observed in the vehicle and the negative control groups. The histologic changes in the experimental group were significantly less than the positive control group. Also, the Blood-brain barrier impermeability was significantly decreased in the other groups compared to the positive control group. Although more studies are needed to verify the exact mechanisms of pretreatment potential effects of omy-miR-۷۳۱ Antagomir, the present results proved that pretreatment potential of omy-miR-۷۳۱ Antagomir can protect the brain tissue against signs of VHS and preferably would be a new approach against VHSV infection in rainbow trout.
کلیدواژه ها:
نویسندگان
N. Zamannejad
ph. D student Shahid Beheshti University
M.R. Bigdeli
Educational Assistant Shahid Beheshti University
A.A. Motallebi
Agricultural research and education organization consultant
H. Kohram
Deputy of the Agricultural Research and Training Organization
A. Haghighi Khiabanian Asl
Deputy of the Agricultural Research and Training Organization
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