The Effect of Serum from Sepsis Patients on The Ex-pression of CXCR۷ Gene in Human Umbilical Cord Blood Mesenchymal Stem Cells In Vitro

Objective: Sepsis is a common cause of mortality in patients admitted to intensive care unit of hospitals. In advanced stage, the disease leads to the death of patients with dysfunction of vital organs such as the heart, kidneys and liver. Mesenchymal stem cells (MSCs) enhance the regenerative capacity of dam-aged tissues by migration and homing in these tissues. The CXCR۷ gene plays an important role in the homing and persis-tence of MSCs in the target tissues.Materials and Methods: For this purpose, the serum of ۲۰ pa-tients with sepsis was collected. Human Umbilical Cord Blood Mesenchymal Stem Cells (hUCB-MSCs) were cultured in plate, four groups were treated with patient’s serum and control group treated with serum from healthy volunteers. After ۲۴ and ۴۸ h, stem cells were trypsinized and extracted RNAs. cDNA was syn-thesized using reverse transcription reaction and special kit.Results: The expression rate of this gene was determined by quantitative Real-Time PCR. The results showed that the ex-pression of CXCR۷ gene in hUCB-MSCs treated with patient’s serums was increased significantly compared to the control group after ۲۴ h. Also, there was a significant increase of this gene expression in ۴۸ h treatment compared to the control and ۲۴ h treatment.Conclusion: High expression of CXCR۷ gene improved effi-ciency of cell therapy to repair damaged tissues caused by sep-sis.