Assessing The Effect of Human Amnion-Derived Mesenchymal Exosomes in Angiogenesis Induction

Objective: Angiogenesis is the creation of new blood vessels from preexisting ones. How blood vessels interact with cells in their nearby microenvironment is yet to be studied but it might be through exosomes. Recently exosomes have gained a lot of attention to be as a carrier for a variety of macromolecules which control intracellular communication. Human amnion mesenchymal tissues (hAm) have various potentials for thera-peutic effects, including angiogenesis. The aim of this study was to evaluate the influence of hAm- mesenchymal-derived exosomes (hAm-Exo) on angiogenesis.Materials and Methods: hAM was isolated from the placenta by enzymatic digestion and cultured into a serum-free ۱۲ well plate. The condition media were collected after ۲۴ hours and their exosomes were isolated using Exocib kit. The exosomes were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and a BCA kit. The angiogenesis activity of hAm-Exo was assessed by the Dorsal Skinfold Chamber in an in vivo angiogenesis model.Results: TEM and DLS showed that exosomes have a size range of about ۳۰ nm with an appearance of cup-shaped. Vessel density, total vessel network length, total branching points and total segments were increased (P<۰.۰۴) by the compositions of hAm-exo. The size of the wound decreased (P<۰.۰۱) compared to the control group.Conclusion: This study demonstrated that condition media from human amnion membrane contained exosomes that can increase angiogenesis when tested in an in vivo angiogenic model. With more studies on this matter hAm-Exo could be a great candidate for the treatment of ischemic diseases.